Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030557_l3w5 AGAAATTCACCATACACTTCTCAGTGTCTTAG(+ACTCAC)GATCCAGATGTCCGGTCCATCCCTGAAACCT NGG 10 8 17 8 24 0 100 5 10
    240605030556_24hq GGGAGAGGTTTCAGGGATGGACCGGACATCTG(+ACTCAC)GATCCTAAGACACTGAGAAGTGTATGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605030555_6mow GTCTGTTCTCCTGAAATCCCAGGATTGGCAGG(+ACTCAC)GTACCTGGCCAGGCCAACCAGGGCCACTCTG NGG 10 8 17 8 24 0 100 5 10
    240605030553_4xr5 GGGTACAGAGTGGCCCTGGTTGGCCTGGCCAG(+ACTCAC)GTACCCTGCCAATCCTGGGATTTCAGGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605030551_yqh0 GCCAACATCTTGGAGCTGGGCTCGTGCCTGAG(+ACTCAC)GTACCAGTCTGCTTTGCTTTGAAACGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605030549_u0uq GTCCTTCCTTCGTTTCAAAGCAAAGCAGACTG(+ACTCAC)GTACCTCAGGCACGAGCCCAGCTCCAAGATG NGG 10 8 17 8 24 0 100 5 10
    240605030548_syt2 GCCATCACCAAAGGGTGCAGCCACAGGGCTGG(+ACTCAC)GTACCTGCATGCTTCATCTCCACCTGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605030547_sxtw AGGATGCCTTCAGGTGGAGATGAAGCATGCAG(+ACTCAC)GTACCCAGCCCTGTGGCTGCACCCTTTGGTG NGG 10 8 17 8 24 0 100 5 10
    240605030544_8xxl GTTTCCAGCTTTAGTACAGATGGCTGCCTGGG(+ACTCAC)GATCCCAGAAGCCTTCCGGACCCTACTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605030543_pyi4 CCCAAAGGAAGTAGGGTCCGGAAGGCTTCTGG(+ACTCAC)GATCCCCAGGCAGCCATCTGTACTAAAGCTG NGG 10 8 17 8 24 0 100 5 10