Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030450_lnsb GGTCTCCAAGAACAGTCCCCTGACAATCTAGG(+ACTCAC)GATCCGGGCGGAGTCTGTCTTGGAAGGGACA NGG 10 8 17 8 24 0 100 5 10
    240605030448_1e7n AACTGTGTCCCTTCCAAGACAGACTCCGCCCG(+ACTCAC)GATCCCTAGATTGTCAGGGGACTGTTCTTGG NGG 10 8 17 8 24 0 100 5 10
    240605030446_76i1 TCAAGGAGAAAGAGGACCCTTGACTCTCCTGG(+ACTCAC)GTACCAGATAAGTCAGGGACACTGTTTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605030445_dfej TTCACACCAAAACAGTGTCCCTGACTTATCTG(+ACTCAC)GTACCCAGGAGAGTCAAGGGTCCTCTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605030443_sx00 CAGCTTGGCTCCTGCTCCCTTGCAACTGAGTG(+ACTCAC)GATCCAGTTTCCTCACAAATTGAACTGACAA NGG 10 8 17 8 24 0 100 5 10
    240605030442_iyzx AGGTGTTGTCAGTTCAATTTGTGAGGAAACTG(+ACTCAC)GATCCACTCAGTTGCAAGGGAGCAGGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605030441_xcw6 AGGTAAACAAGGGAAGGACTGATTTCCTGTTG(+ACTCAC)GATCCAGCAAACAGTGATATGATCCTCACAC NGG 10 8 17 8 24 0 100 5 10
    240605030440_ipjw TTCATGTGTGAGGATCATATCACTGTTTGCTG(+ACTCAC)GATCCAACAGGAAATCAGTCCTTCCCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605030439_5xfn ACTCCACATGTATGCACACGATGCAGGAATTG(+ACTCAC)GATCCAGTTCAGGAGTTCAAGCTCTGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605030437_q0c0 TGAAGCTGGACAGAGCTTGAACTCCTGAACTG(+ACTCAC)GATCCAATTCCTGCATCGTGTGCATACATGT NGG 10 8 17 8 24 0 100 5 10