Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030522_smb0 ACTCCACCCACACTTGTGGGAACTCCAAAAGG(+ACTCAC)GTACCTGGCCCACAAGATTCTGGGGTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240605030520_mqot CCTATTTCTGACCCCAGAATCTTGTGGGCCAG(+ACTCAC)GTACCCTTTTGGAGTTCCCACAAGTGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605030519_2t1h CGGGCTGTATTTAAAGAGCCATACTCAAGAAG(+ACTCAC)GTACCAAGTCTGCGCAACCACACTGCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605030518_6poa TCTGGGCTCTGCAGTGTGGTTGCGCAGACTTG(+ACTCAC)GTACCTTCTTGAGTATGGCTCTTTAAATACA NGG 10 8 17 8 24 0 100 5 10
    240605030517_x96w CTACTTAGGCCGTCAACTACAGGGAGAAGATG(+ACTCAC)GTACCCTGCCAGAGTGGGAGGGAGGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605030515_re0v CTAAGCTCTCTCCTCCCTCCCACTCTGGCAGG(+ACTCAC)GTACCATCTTCTCCCTGTAGTTGACGGCCTA NGG 10 8 17 8 24 0 100 5 10
    240605030513_r0ar CCAGATGAAGCCCTTGGGGAACATCTGCCAGG(+ACTCAC)GATCCGCATGGTGTTGGCTCCATCCATTGCT NGG 10 8 17 8 24 0 100 5 10
    240605030512_aqje ACTAAAGCAATGGATGGAGCCAACACCATGCG(+ACTCAC)GATCCCTGGCAGATGTTCCCCAAGGGCTTCA NGG 10 8 17 8 24 0 100 5 10
    240605030510_rb7k TTAGAGCTTTTTTTAAAAACCAAGTATTTGGG(+ACTCAC)GATCCTCAGCAGAGGTATTCATACAGACTCC NGG 10 8 17 8 24 0 100 5 10
    240605030508_hmos CTTTGGGAGTCTGTATGAATACCTCTGCTGAG(+ACTCAC)GATCCCCAAATACTTGGTTTTTAAAAAAAGC NGG 10 8 17 8 24 0 100 5 10