Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030419_vygy TGCCTGACGGAGCTTTGGGCGCAGCTGGAAAG(+ACTCAC)GATCCGTAGAGCAGTGGCTTCCCTTGTATGC NGG 10 8 17 8 24 0 100 5 10
    240605030418_0ya6 TGCACGCATACAAGGGAAGCCACTGCTCTACG(+ACTCAC)GATCCTTTCCAGCTGCGCCCAAAGCTCCGTC NGG 10 8 17 8 24 0 100 5 10
    240605030416_tho8 GGTGCCTCTCTCCCCCCTTAGACATCAGACAG(+ACTCAC)GATCCCTTTATAGAATAGCCAAGTAAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240605030415_lcpz CACTGGTTTCTTACTTGGCTATTCTATAAAGG(+ACTCAC)GATCCTGTCTGATGTCTAAGGGGGGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605030413_dr91 CAGCACTGCTCCCTGGGTGCTGAGCACTGGTG(+ACTCAC)GATCCAGTGCCCCAGAGGGCAAGTCCCTTCG NGG 10 8 17 8 24 0 100 5 10
    240605030412_0vry AGCTGCGAAGGGACTTGCCCTCTGGGGCACTG(+ACTCAC)GATCCACCAGTGCTCAGCACCCAGGGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605030411_b4ia AAAACCATCAAGATGCAATGGTCTTTATTAAG(+ACTCAC)GTACCTGCGGTCAGCTGGCAACCATCTCTTG NGG 10 8 17 8 24 0 100 5 10
    240605030409_2ju9 TGTTTCAAGAGATGGTTGCCAGCTGACCGCAG(+ACTCAC)GTACCTTAATAAAGACCATTGCATCTTGATG NGG 10 8 17 8 24 0 100 5 10
    240605030407_3lxk GTAGCGTCTCTGGAGGCCAGAAGATGGCATTG(+ACTCAC)GATCCCCGGAACTGGAGTCACAAGTTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605030405_ioia TGGAGCTCACAACTTGTGACTCCAGTTCCGGG(+ACTCAC)GATCCAATGCCATCTTCTGGCCTCCAGAGAC NGG 10 8 17 8 24 0 100 5 10