Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030345_omdl CCATGGCCCCTTGGTATCTGTGGGATGCTGGG(+ACTCAC)GATCCGAATCCTGGCCCTCATGCTTGGACAG NGG 10 8 17 8 24 0 100 5 10
    240605030344_7xnu GCTTGCTGTCCAAGCATGAGGGCCAGGATTCG(+ACTCAC)GATCCCCAGCATCCCACAGATACCAAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605030342_f4oq AAGATCAGCACTCCTGCTTGCCTGACTTGCTG(+ACTCAC)GATCCCAAGGGCACTCACTGAACCACCCTCC NGG 10 8 17 8 24 0 100 5 10
    240605030340_cqq6 TTATGGGAGGGTGGTTCAGTGAGTGCCCTTGG(+ACTCAC)GATCCAGCAAGTCAGGCAAGCAGGAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605030339_n0n6 TCTGCAGTCTGTCTCCGGCCTGGGGACACATG(+ACTCAC)GTACCTTCAAAGTAGTTGGAATCTGAGCATC NGG 10 8 17 8 24 0 100 5 10
    240605030338_2d1e AGCGTGATGCTCAGATTCCAACTACTTTGAAG(+ACTCAC)GTACCATGTGTCCCCAGGCCGGAGACAGACT NGG 10 8 17 8 24 0 100 5 10
    240605030335_58tm AGTCAGGGTAGGACCAGCTCCGGTTGTTGGGG(+ACTCAC)GATCCGATGAAGACCAGCCTGTACATCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605030334_8mej TATGTAGCAGATGTACAGGCTGGTCTTCATCG(+ACTCAC)GATCCCCCAACAACCGGAGCTGGTCCTACCC NGG 10 8 17 8 24 0 100 5 10
    240605030333_focp GAGCTTGCGCTTGCTTGCTAAACCTCTAACAG(+ACTCAC)GTACCTGGGGATGATGGTGCACACCTATAAC NGG 10 8 17 8 24 0 100 5 10
    240605030329_3bbm TTAGGGTTATAGGTGTGCACCATCATCCCCAG(+ACTCAC)GTACCTGTTAGAGGTTTAGCAAGCAAGCGCA NGG 10 8 17 8 24 0 100 5 10