Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030328_bbw6 GGACAAAAAAAAAAGAAAAGAAAAGAAAAAAG(+ACTCAC)GATCCCCCCCTTCTGTTTTTGTTCCTCGTGA NGG 10 8 17 8 24 0 100 5 10
    240605030326_aw2i AGAAATCACGAGGAACAAAAACAGAAGGGGGG(+ACTCAC)GATCCTTTTTTCTTTTCTTTTCTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605030324_5yfv AGTTGATAAGTTTTCTGATATTCTGATATTAG(+ACTCAC)GTACCACGGATGCAGGAATATTACTTGATAT NGG 10 8 17 8 24 0 100 5 10
    240605030322_wl8q AGCCAATATCAAGTAATATTCCTGCATCCGTG(+ACTCAC)GTACCTAATATCAGAATATCAGAAAACTTAT NGG 10 8 17 8 24 0 100 5 10
    240605030320_nwyf GCACCTGCTTGGCTGTGACTCAGGTCCCAGGG(+ACTCAC)GATCCAGATCCCTTCTCTGACCTTTGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240605030319_2mld CTGGTGCCAGCAAAGGTCAGAGAAGGGATCTG(+ACTCAC)GATCCCCTGGGACCTGAGTCACAGCCAAGCA NGG 10 8 17 8 24 0 100 5 10
    240605030317_lkel GGTCTTTTGAGAGATTTATTGAATAAGAACAG(+ACTCAC)GTACCAGCGGGACACTGAAACAGTTATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605030315_htdp GCACACAGCATAACTGTTTCAGTGTCCCGCTG(+ACTCAC)GTACCTGTTCTTATTCAATAAATCTCTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605030314_ifp3 ATGGATATTATATCAGACCTCCTTCTGGTCAG(+ACTCAC)GATCCAGTCTACCACACTTATGACTAAGCGA NGG 10 8 17 8 24 0 100 5 10
    240605030313_8trm TGATATCGCTTAGTCATAAGTGTGGTAGACTG(+ACTCAC)GATCCTGACCAGAAGGAGGTCTGATATAATA NGG 10 8 17 8 24 0 100 5 10