Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030312_qdjq TCAGGGTGTTATCCTCTTCCTGAGACACTCAG(+ACTCAC)GATCCTTGGAAGAACAACTTTTGCTGTCCGT NGG 10 8 17 8 24 0 100 5 10
    240605030310_ca13 GATCCACGGACAGCAAAAGTTGTTCTTCCAAG(+ACTCAC)GATCCTGAGTGTCTCAGGAAGAGGATAACAC NGG 10 8 17 8 24 0 100 5 10
    240605030308_o4l7 GTTTCTTTCCGAGCTGATGACAAGACTGAATG(+ACTCAC)GTACCTTTTCTTTCTGATCGGTTCTAAACAT NGG 10 8 17 8 24 0 100 5 10
    240605030307_s10o GGCCAATGTTTAGAACCGATCAGAAAGAAAAG(+ACTCAC)GTACCATTCAGTCTTGTCATCAGCTCGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605030306_13gz CCCCAAGACTTTGTTTTATCGGAGAGGAAAAG(+ACTCAC)GTACCCCATTTCATTCATTGGTGTAAGGATG NGG 10 8 17 8 24 0 100 5 10
    240605030304_7pk8 GAAAACATCCTTACACCAATGAATGAAATGGG(+ACTCAC)GTACCTTTTCCTCTCCGATAAAACAAAGTCT NGG 10 8 17 8 24 0 100 5 10
    240605030302_hxkn AACTTAAATAAAAATAGCACCAGAAGGACCAG(+ACTCAC)GTACCAATAACATATTTCACCCATCCAGTAA NGG 10 8 17 8 24 0 100 5 10
    240605030301_e2kt TAAAATTACTGGATGGGTGAAATATGTTATTG(+ACTCAC)GTACCTGGTCCTTCTGGTGCTATTTTTATTT NGG 10 8 17 8 24 0 100 5 10
    240605030259_mpbm TACAAACTAATTTTTAGTAGCTGATAATACAG(+ACTCAC)GATCCCACAGTCTATTTACATAAAAGCCCGT NGG 10 8 17 8 24 0 100 5 10
    240605030258_s4nw TAAAAACGGGCTTTTATGTAAATAGACTGTGG(+ACTCAC)GATCCTGTATTATCAGCTACTAAAAATTAGT NGG 10 8 17 8 24 0 100 5 10