Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030751_3x2x AGAAATACAGAAATGGAAGTCAAACCGCCGAG(+ACTCAC)GATCCTGCGGCGGTGACCTCGGGGCTCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605030749_6ulk CTTCGTCCAGAGCCCCGAGGTCACCGCCGCAG(+ACTCAC)GATCCTCGGCGGTTTGACTTCCATTTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240605030747_5pf5 CAGAGTCCGCTGCCTTATCTATCAGCAGAAGG(+ACTCAC)GATCCAGGTGGTTCTTGGAACTCAGATAAGC NGG 10 8 17 8 24 0 100 5 10
    240605030745_hpyl GGGAAGCTTATCTGAGTTCCAAGAACCACCTG(+ACTCAC)GATCCCTTCTGCTGATAGATAAGGCAGCGGA NGG 10 8 17 8 24 0 100 5 10
    240605030744_abgs GCTTGCTGTGCAGGCATGAGGGTGTACACTTG(+ACTCAC)GATCCCCAGAACCCACATAATAGCTGGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605030742_jeld CAGCTGTGCCCAGCTATTATGTGGGTTCTGGG(+ACTCAC)GATCCAAGTGTACACCCTCATGCCTGCACAG NGG 10 8 17 8 24 0 100 5 10
    240605030740_c54h CTAATTCTCCCCGATTTTAGCATCCTCCGAAG(+ACTCAC)GATCCCATCTTTGAAAACGGCAGAAAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240605030739_4h0h GTCTTTGCACTTTCTGCCGTTTTCAAAGATGG(+ACTCAC)GATCCTTCGGAGGATGCTAAAATCGGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605030738_r15b CTTGACTTCATCTTTCATCTGAGGAGTTTCTG(+ACTCAC)GATCCTCAACTGGGTCGACTTTCTCTGATGG NGG 10 8 17 8 24 0 100 5 10
    240605030736_8ng5 AGGAGCCATCAGAGAAAGTCGACCCAGTTGAG(+ACTCAC)GATCCAGAAACTCCTCAGATGAAAGATGAAG NGG 10 8 17 8 24 0 100 5 10