Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605031307_fu9y TCCAAAGAGTGGCCAGGAGGATGGGCCAAAGG(+ACTCAC)GATCCTTGTGAGAGGAATGAAATCATCCAAG NGG 10 8 17 8 24 0 100 5 10
    240605031306_rns2 GTTCAGTCCCCAGGTCAGAAGGGGAGAGTGTG(+ACTCAC)GATCCTGACATGGTTCTATACTCTTCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605031305_xl5d ATTACCCCCAGAAGAGTATAGAACCATGTCAG(+ACTCAC)GATCCACACTCTCCCCTTCTGACCTGGGGAC NGG 10 8 17 8 24 0 100 5 10
    240605031304_npqp GTGACCATTTTTTACACAACCATACTGGAAAG(+ACTCAC)GATCCAGTACCTTTTTTTTTTTAGTGTGATT NGG 10 8 17 8 24 0 100 5 10
    240605031302_v7s9 TCTGAAATCACACTAAAAAAAAAAAGGTACTG(+ACTCAC)GATCCTTTCCAGTATGGTTGTGTAAAAAATG NGG 10 8 17 8 24 0 100 5 10
    240605031300_9e5d TCTTTTCATCAAAAACCTTATCATTTCCTATG(+ACTCAC)GTACCATTGTCTGTCTCCAGAGTGTGTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605031259_m6yh CAGATTTTGACACACTCTGGAGACAGACAATG(+ACTCAC)GTACCATAGGAAATGATAAGGTTTTTGATGA NGG 10 8 17 8 24 0 100 5 10
    240605031258_tqlo ACTGTGTAGCCTAGGTTGATATCAAATCAATG(+ACTCAC)GTACCCTCAGCACACATACCAGTTCAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605031256_2z04 TAACTGAGGCTGAACTGGTATGTGTGCTGAGG(+ACTCAC)GTACCATTGATTTGATATCAACCTAGGCTAC NGG 10 8 17 8 24 0 100 5 10
    240605031255_hbhm TAGACAAGCCACTTGTTGTATCTGAGTTTTAG(+ACTCAC)GTACCAGAGGGGAAATACTGCCTGATCGATT NGG 10 8 17 8 24 0 100 5 10