Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030821_duwb TTGAACACTAGAGGGTTTCTTTCTAAGAGATG(+ACTCAC)GTACCTAACCCTGTAACCAAGGAACTGTTTG NGG 10 8 17 8 24 0 100 5 10
    240605030820_6z21 TTTTCCAAACAGTTCCTTGGTTACAGGGTTAG(+ACTCAC)GTACCATCTCTTAGAAAGAAACCCTCTAGTG NGG 10 8 17 8 24 0 100 5 10
    240605030819_myqz TTCAACCCAAGGAAAAAGGTTGTGATTATTAG(+ACTCAC)GTACCAGGCGTAAATAGCAAGGAAGAAATGT NGG 10 8 17 8 24 0 100 5 10
    240605030817_hgh7 CTATGACATTTCTTCCTTGCTATTTACGCCTG(+ACTCAC)GTACCTAATAATCACAACCTTTTTCCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605030816_x952 TTGAACACTAGAGGGTTTCTTTCTAAGAGATG(+ACTCAC)GTACCTAACCCTGTAACCAAGGAACTGTTTG NGG 10 8 17 8 24 0 100 5 10
    240605030815_j1ql TTTTCCAAACAGTTCCTTGGTTACAGGGTTAG(+ACTCAC)GTACCATCTCTTAGAAAGAAACCCTCTAGTG NGG 10 8 17 8 24 0 100 5 10
    240605030814_o5gk GATTTCCAAGGCAGTTAATTATGAAAACTGTG(+ACTCAC)GATCCTGGCCAGTGCTGGCCATTGGATCTCC NGG 10 8 17 8 24 0 100 5 10
    240605030812_wfks AAGGTGGAGATCCAATGGCCAGCACTGGCCAG(+ACTCAC)GATCCACAGTTTTCATAATTAACTGCCTTGG NGG 10 8 17 8 24 0 100 5 10
    240605030810_gww9 GCTCATAACTATCTGTAGCTCCAGTCCCAGAG(+ACTCAC)GATCCGACAGCCTCACACAGACAACGTCAAT NGG 10 8 17 8 24 0 100 5 10
    240605030809_awxb CAGTCATTGACGTTGTCTGTGTGAGGCTGTCG(+ACTCAC)GATCCTCTGGGACTGGAGCTACAGATAGTTA NGG 10 8 17 8 24 0 100 5 10