Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030658_brqw GACAATGAGGACATCTGCCTCCCCTCTGGAAG(+ACTCAC)GTACCTCTCGGGTGAGGCGGCTTAGGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605030657_cbf5 TCAAAATATAACTAAATACTGGTAACAATTTG(+ACTCAC)GTACCCAGCACTCTTAGAAGAGAACAGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605030655_oa0u ATTGGCAACCTGTTCTCTTCTAAGAGTGCTGG(+ACTCAC)GTACCAAATTGTTACCAGTATTTAGTTATAT NGG 10 8 17 8 24 0 100 5 10
    240605030654_y2pl AGCAGACACTGTGCCCTTAGCCTGCTGGCAAG(+ACTCAC)GTACCCAAGAAGATGGAGGACTTTGAAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605030652_r8q2 GCTCCTGGCTTCAAAGTCCTCCATCTTCTTGG(+ACTCAC)GTACCTTGCCAGCAGGCTAAGGGCACAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605030650_a861 GAAAGGGAAGAGCAGGAATGCCCTGGGCCCGG(+ACTCAC)GATCCCCGCATTGCACGTTGCATGCGGATGG NGG 10 8 17 8 24 0 100 5 10
    240605030648_a2hi ATGGTCCATCCGCATGCAACGTGCAATGCGGG(+ACTCAC)GATCCCGGGCCCAGGGCATTCCTGCTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605030646_fazm GTCTGCCTTCAGCTCCCAACCCTAACCTCCTG(+ACTCAC)GATCCCCAAGCCATGTTTCTCCCACAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605030644_m90z TGCATCCACCTGTGGGAGAAACATGGCTTGGG(+ACTCAC)GATCCAGGAGGTTAGGGTTGGGAGCTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605030642_ryxc CACAGAGCATCTCAGTTGGACTGGACCACCAG(+ACTCAC)GATCCCCAGAGCCAGCCTTCCCCACCTCCCC NGG 10 8 17 8 24 0 100 5 10