Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030156_5fj4 GCCTCCCTGCACTGGAGCAAGGGCCACCACGG(+ACTCAC)GTACCAGGGTTTAGCCTTTTGAGAGCAGCAT NGG 10 8 17 8 24 0 100 5 10
    240605030155_hj1b TTCCCACCAAGCTAGGGCGTGGCTAGATAAAG(+ACTCAC)GTACCTCTGACTTGGGTTTCTAACAGCGTGA NGG 10 8 17 8 24 0 100 5 10
    240605030154_3coh ATGTATCACGCTGTTAGAAACCCAAGTCAGAG(+ACTCAC)GTACCTTTATCTAGCCACGCCCTAGCTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605030153_km0z AGTTTAAACCTACATACCCAGTTTCCTTTCAG(+ACTCAC)GTACCTTGACCTGCTTCCAGGGGAACTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605030152_wirz GCTATTAAAAGTTCCCCTGGAAGCAGGTCAAG(+ACTCAC)GTACCTGAAAGGAAACTGGGTATGTAGGTTT NGG 10 8 17 8 24 0 100 5 10
    240605030150_c68g TTCCAAGGATGGAACTCAGGTGGTTAGTATTG(+ACTCAC)GTACCAGGAACCTAAACTTACCCTTTGAACC NGG 10 8 17 8 24 0 100 5 10
    240605030149_blzg GGGTTGGTTCAAAGGGTAAGTTTAGGTTCCTG(+ACTCAC)GTACCAATACTAACCACCTGAGTTCCATCCT NGG 10 8 17 8 24 0 100 5 10
    240605030147_cts7 AATGCCCTTTCGTGCTTTGTCACCTACACTCG(+ACTCAC)GTACCCAGGACCTGGTGCTATTAACTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605030145_hexm GAAGGCACAGAGTTAATAGCACCAGGTCCTGG(+ACTCAC)GTACCGAGTGTAGGTGACAAAGCACGAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605030144_krg0 TCAGGGAAGTATGTGTCTCCCGGGGAGGGATG(+ACTCAC)GTACCTCCGTGGGCATCCCCCAAAGGCCCTC NGG 10 8 17 8 24 0 100 5 10