Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605030142_aeah TGGTGGAGGGCCTTTGGGGGATGCCCACGGAG(+ACTCAC)GTACCATCCCTCCCCGGGAGACACATACTTC NGG 10 8 17 8 24 0 100 5 10
    240605030141_eykp CTCCACTTTATTTTTGAAATGCATACTTATTG(+ACTCAC)GTACCCTTTTGTTTCTTCCCTCACAAAAATG NGG 10 8 17 8 24 0 100 5 10
    240605030139_ki8a GGCAACATTTTTGTGAGGGAAGAAACAAAAGG(+ACTCAC)GTACCAATAAGTATGCATTTCAAAAATAAAG NGG 10 8 17 8 24 0 100 5 10
    240605030138_q6wm AATAGTTTCTGTATTTTCTAATTATGAATAGG(+ACTCAC)GTACCCTTGATGTCTTCAAAGTTTTGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605030136_mtt9 TTAAGTGAGACAAAACTTTGAAGACATCAAGG(+ACTCAC)GTACCCTATTCATAATTAGAAAATACAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605030134_bifq TTCTATAAAACTGAGTTTGCTGAAACTTCCCG(+ACTCAC)GTACCCCACCATGCTTCTCTAATAAGCATGA NGG 10 8 17 8 24 0 100 5 10
    240605030132_vfum TGAGCTCATGCTTATTAGAGAAGCATGGTGGG(+ACTCAC)GTACCGGGAAGTTTCAGCAAACTCAGTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605030130_8rd1 TCTCTGAGCAAAGAGGCTACAAGGGAGTTTTG(+ACTCAC)GATCCCGTGAGGCAGGCGCTACAGATGGAAC NGG 10 8 17 8 24 0 100 5 10
    240605030129_uj0v TCCCAGTTCCATCTGTAGCGCCTGCCTCACGG(+ACTCAC)GATCCAAAACTCCCTTGTAGCCTCTTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240605030127_zqff GCAATCATAGCTTGCATTTTGCACATACACAG(+ACTCAC)GATCCGAGCCAGCTAGTGTTATGTCAATCTG NGG 10 8 17 8 24 0 100 5 10