Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613163006_fcuo GCCTCTCTCTCTCTCTTGGCCTCTCCTGTCTG(+AGGACA)GATCCTTTATAGAACTGAGGTGGGGAGCAGC NGG 10 8 17 8 24 0 100 5 10
    240613163004_okwz TCCCAAAGAATGAAGCAATCTATGAAGAGAGG(+AGGACA)GTACCAGACAAAGATCCAAGACACCAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240613163003_qr0b GGATTTGGGCTGGTGTCTTGGATCTTTGTCTG(+AGGACA)GTACCCTCTCTTCATAGATTGCTTCATTCTT NGG 10 8 17 8 24 0 100 5 10
    240613163002_rbgf CATTACTTTTTTCCTGTATATGAAGAAAATTG(+AGGACA)GTACCCTGTACTTACAATTTATTTATGTTAT NGG 10 8 17 8 24 0 100 5 10
    240613163000_b1xd ATGCAATAACATAAATAAATTGTAAGTACAGG(+AGGACA)GTACCAATTTTCTTCATATACAGGAAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240613162957_oeci AAGCATAGATGTAATATAAATTTGATTCCCAG(+AGGACA)GATCCTAGACATCTTAGATAAGTCAATACAA NGG 10 8 17 8 24 0 100 5 10
    240613162956_lsbz TTTCTTTGTATTGACTTATCTAAGATGTCTAG(+AGGACA)GATCCTGGGAATCAAATTTATATTACATCTA NGG 10 8 17 8 24 0 100 5 10
    240613162955_xsu0 GAAGAAACGGGGGAGACCTTGAATGTTAACAG(+AGGACA)GATCCATGGTGAGGGTGTTGGGGTTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240613162953_ej1x ATTTAACACACAACCCCAACACCCTCACCATG(+AGGACA)GATCCTGTTAACATTCAAGGTCTCCCCCGTT NGG 10 8 17 8 24 0 100 5 10
    240613162952_nugh CCTCCGTGTCGTGCAGACTCAGAATAGACTTG(+AGGACA)GATCCTTTCTCTCTGATTCTCTGGTGCCCAC NGG 10 8 17 8 24 0 100 5 10