Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162915_n7ij CTGGCCTCTTGCTTAGCACACTGCCGGGTCAG(+AGGACA)GATCCCTACGCGGGACTACTGGGAACAAAGT NGG 10 8 17 8 24 0 100 5 10
    240613162914_xqar CCGAGGGGGAAATTATGTCCACGGCCTCGCTG(+AGGACA)GTACCATGGAAGACTCATGCCCCCTGCCCAT NGG 10 8 17 8 24 0 100 5 10
    240613162912_zqio GGGAAATGGGCAGGGGGCATGAGTCTTCCATG(+AGGACA)GTACCAGCGAGGCCGTGGACATAATTTCCCC NGG 10 8 17 8 24 0 100 5 10
    240613162911_lgje GCACATGGATCTCACCCGTATTCTGTTGTATG(+AGGACA)GTACCTCGGTACATCTTTGTGGTTGTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240613162909_637n CAGTTTTAACACAACCACAAAGATGTACCGAG(+AGGACA)GTACCATACAACAGAATACGGGTGAGATCCA NGG 10 8 17 8 24 0 100 5 10
    240613162907_p43q GAGTAAATAGGGGACATGGTGCATTTTTGCTG(+AGGACA)GATCCTGTGAAAGGTTTATTTAGAGCCGATT NGG 10 8 17 8 24 0 100 5 10
    240613162906_ydww TCTGGAATCGGCTCTAAATAAACCTTTCACAG(+AGGACA)GATCCAGCAAAAATGCACCATGTCCCCTATT NGG 10 8 17 8 24 0 100 5 10
    240613162905_s2bn TAGTTTTGAGGGCTTACACAAGCTTATTCTTG(+AGGACA)GTACCACAGGAATTTGGTTAAATGGAATTTC NGG 10 8 17 8 24 0 100 5 10
    240613162903_0pfm CAAAGGAAATTCCATTTAACCAAATTCCTGTG(+AGGACA)GTACCAAGAATAAGCTTGTGTAAGCCCTCAA NGG 10 8 17 8 24 0 100 5 10
    240613162902_z5do GCATTCCCCGCTGCAGTCACCTCATAGGCTTG(+AGGACA)GATCCATCAAGACCTACTTCAGGCCCCGGCT NGG 10 8 17 8 24 0 100 5 10