Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162806_8huy TGTCCCCATGAAAGATGATGAAAGCCACCATG(+AGGACA)GATCCTCGCTGCCGAGTGGTGGGAGGGTTAG NGG 10 8 17 8 24 0 100 5 10
    240613162805_lm06 GTTAGAATCACAACCAACTCTGTAATAAATGG(+AGGACA)GATCCTTTAACTCCTGACCTACTAAAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240613162803_bdwf TCTTGGGGGCTTTAGTAGGTCAGGAGTTAAAG(+AGGACA)GATCCCATTTATTACAGAGTTGGTTGTGATT NGG 10 8 17 8 24 0 100 5 10
    240613162802_5ypp ATGCGTGAGAACCCAATGTGAGAACAGGGAAG(+AGGACA)GATCCTTTCTGATTGTTGTGGCCTTGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240613162800_6qd0 AAATAAAACCCAAGGCCACAACAATCAGAAAG(+AGGACA)GATCCTTCCCTGTTCTCACATTGGGTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240613162759_6cob TGACTAACATACTTGAGGGAGGACAGTGAAGG(+AGGACA)GATCCTTAGGTCACCTACTCCTTGGTTGGAC NGG 10 8 17 8 24 0 100 5 10
    240613162757_gj7z CACTTGTCCAACCAAGGAGTAGGTGACCTAAG(+AGGACA)GATCCCTTCACTGTCCTCCCTCAAGTATGTT NGG 10 8 17 8 24 0 100 5 10
    240613162755_twdn CTCTGAGGGGTCTGCGCAGGTTTGGGCTACTG(+AGGACA)GATCCCTGGGGTTGATCAAGCTGCGTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613162752_5rz5 GCCCCCTGGAACGCAGCTTGATCAACCCCAGG(+AGGACA)GATCCAGTAGCCCAAACCTGCGCAGACCCCT NGG 10 8 17 8 24 0 100 5 10
    240613162751_fvfo ATACAGTGCCTTCACCATGGTCACAAATTCTG(+AGGACA)GTACCTTTGTGCAAAAGTTGTGGTTGGTTTT NGG 10 8 17 8 24 0 100 5 10