Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162704_39of TAGGATGATGTGTGGCTCGCAACTGGAAAGGG(+AGGACA)GTACCTGCAATGTTTGCACAGCCTAAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240613162702_7uaq AGGGATGTCAGATAACTTGACCCAGGGCTATG(+AGGACA)GATCCACTGTGAAGGATGGCTTCCCAGAGTC NGG 10 8 17 8 24 0 100 5 10
    240613162701_azxw CAGAAGACTCTGGGAAGCCATCCTTCACAGTG(+AGGACA)GATCCATAGCCCTGGGTCAAGTTATCTGACA NGG 10 8 17 8 24 0 100 5 10
    240613162700_b0o0 TTTCTGGGCTCTGCTGGAAGGGCCCTGAGATG(+AGGACA)GTACCCGACCTGTGGAGACTGAGAGGGTACT NGG 10 8 17 8 24 0 100 5 10
    240613162658_2jl8 GTAACAGTACCCTCTCAGTCTCCACAGGTCGG(+AGGACA)GTACCATCTCAGGGCCCTTCCAGCAGAGCCC NGG 10 8 17 8 24 0 100 5 10
    240613162656_sjyw GTGTGGCCAGTGCCAAGACCACCGTCTCTTGG(+AGGACA)GATCCTCTGTCTTTATCTTTTGTTCGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240613162655_v6lz ATGAAACAGACGAACAAAAGATAAAGACAGAG(+AGGACA)GATCCCAAGAGACGGTGGTCTTGGCACTGGC NGG 10 8 17 8 24 0 100 5 10
    240613162653_7w1y CCCGAGCCCTTCACGGGCATCCGAGGGAAAGG(+AGGACA)GTACCAGAAGCATTCCCTCTTAATCATTGCA NGG 10 8 17 8 24 0 100 5 10
    240613162652_s546 ATGTCTGCAATGATTAAGAGGGAATGCTTCTG(+AGGACA)GTACCCTTTCCCTCGGATGCCCGTGAAGGGC NGG 10 8 17 8 24 0 100 5 10
    240613162651_y4w6 GAGAACAGAACAGAACTTCAGAGATCAAAGTG(+AGGACA)GATCCGAGGGTAGATGTACCTTAGCCCAGAG NGG 10 8 17 8 24 0 100 5 10