Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162734_8oqv AGACCACCAGAGGAAGCAGCCTTCCTGCAAGG(+AGGACA)GATCCATTTCTGGACCTCTCCCTATTTAGGA NGG 10 8 17 8 24 0 100 5 10
    240613162733_ahu9 CAAAGCCAAGGTTCATTCGGTGGAAGTTTTAG(+AGGACA)GTACCTCAAGGCAGTTCTGGAAAGTGTACCC NGG 10 8 17 8 24 0 100 5 10
    240613162732_bbxw AACTGGGGTACACTTTCCAGAACTGCCTTGAG(+AGGACA)GTACCTAAAACTTCCACCGAATGAACCTTGG NGG 10 8 17 8 24 0 100 5 10
    240613162730_z2d8 GAATACAAAGACAGCTGCTTGTTTTCTCCTTG(+AGGACA)GTACCTTAAAAATGAGTAAGTGCCACGTCTG NGG 10 8 17 8 24 0 100 5 10
    240613162729_ghxv TTCTCCAGACGTGGCACTTACTCATTTTTAAG(+AGGACA)GTACCAAGGAGAAAACAAGCAGCTGTCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613162727_pziz CTCTAACCTATGACTCTAATAACATCATAATG(+AGGACA)GATCCCAAATCCTTATGCTCAGGAGGCCCAG NGG 10 8 17 8 24 0 100 5 10
    240613162726_nkkj ATCTGCTGGGCCTCCTGAGCATAAGGATTTGG(+AGGACA)GATCCATTATGATGTTATTAGAGTCATAGGT NGG 10 8 17 8 24 0 100 5 10
    240613162724_4ne6 TTACCGTCACCCTCTCAGAACCCTCCCTGCTG(+AGGACA)GTACCTACCCTCTATTTATTAGCCGACTCGT NGG 10 8 17 8 24 0 100 5 10
    240613162723_0rw7 CAGGGACGAGTCGGCTAATAAATAGAGGGTAG(+AGGACA)GTACCAGCAGGGAGGGTTCTGAGAGGGTGAC NGG 10 8 17 8 24 0 100 5 10
    240613162721_cm4i TGGCTCAGTAGACCCAAACCAGGACTGTCCTG(+AGGACA)GATCCACTTTAGTCATGCTACCAGTGGGCTG NGG 10 8 17 8 24 0 100 5 10