Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162824_4mwo CCAGCATGGATGGCACCATTCCCTAGGCAGAG(+AGGACA)GATCCTGGGGCCCAGAAGAGTAGAGAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240613162822_crvn CAGGCCCAGTGTCACTCTTCTTGCTGCCTTTG(+AGGACA)GATCCAGATGTGAATTCTCTGCTACCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240613162820_gv0a GTGCTGGAGAGGTAGCAGAGAATTCACATCTG(+AGGACA)GATCCAAAGGCAGCAAGAAGAGTGACACTGG NGG 10 8 17 8 24 0 100 5 10
    240613162818_ddd6 ACTTTTGAGGCCACTGCCAGGACCCCAGCCTG(+AGGACA)GATCCGGGTTTTATCCAGGGACACCCAGCTA NGG 10 8 17 8 24 0 100 5 10
    240613162816_1i84 CTTCATAGCTGGGTGTCCCTGGATAAAACCCG(+AGGACA)GATCCAGGCTGGGGTCCTGGCAGTGGCCTCA NGG 10 8 17 8 24 0 100 5 10
    240613162814_qvf3 CATTCCTGCCAGTTTGGCTAGCTCTAGATAGG(+AGGACA)GATCCTGCCTTGTATCCTGAGACCCAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240613162813_xk4o TCTGGTCCTCTGGGTCTCAGGATACAAGGCAG(+AGGACA)GATCCCTATCTAGAGCTAGCCAAACTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240613162811_9kt0 GAAGGAATCATACTAGAGAAGAAAAGTCAGAG(+AGGACA)GTACCACAATCAATAAGAAAACAGTAAATTA NGG 10 8 17 8 24 0 100 5 10
    240613162810_m5xi GTTTGTAATTTACTGTTTTCTTATTGATTGTG(+AGGACA)GTACCTCTGACTTTTCTTCTCTAGTATGATT NGG 10 8 17 8 24 0 100 5 10
    240613162809_4rhb GGGCGCTAACCCTCCCACCACTCGGCAGCGAG(+AGGACA)GATCCATGGTGGCTTTCATCATCTTTCATGG NGG 10 8 17 8 24 0 100 5 10