Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162841_xh06 CAACTTCTTTCCAAACCTCCAGACAGAGAGAG(+AGGACA)GTACCTGGTTTAGCAGCCCTGGCGGGCTCCC NGG 10 8 17 8 24 0 100 5 10
    240613162840_8zl1 AGTTCAGATGGGAAATTTAGGAGAGTTTTTGG(+AGGACA)GTACCCGAGTTACTTCACATTATGTAGTACC NGG 10 8 17 8 24 0 100 5 10
    240613162838_0fma TTTGAGGTACTACATAATGTGAAGTAACTCGG(+AGGACA)GTACCCAAAAACTCTCCTAAATTTCCCATCT NGG 10 8 17 8 24 0 100 5 10
    240613162836_0qzm TTTTTCCACAGGATAAAGAACATCCAATTTGG(+AGGACA)GTACCAGAAAGGCTGACATGAAAATTGCTTA NGG 10 8 17 8 24 0 100 5 10
    240613162835_sn6j TAAGATAAGCAATTTTCATGTCAGCCTTTCTG(+AGGACA)GTACCCAAATTGGATGTTCTTTATCCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240613162832_d4v1 GTGTATATAAGCAGTCCTCCCTGGGTACCCGG(+AGGACA)GATCCCTGTAGCAGCATCGAGGCGTTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240613162831_gr5q TTATTGCAGGAACGCCTCGATGCTGCTACAGG(+AGGACA)GATCCCGGGTACCCAGGGAGGACTGCTTATA NGG 10 8 17 8 24 0 100 5 10
    240613162829_tkkt CCATCCATGCTGGGTGCCATCCCACCTCAATG(+AGGACA)GTACCAATATAACCCCCAACAGACATTTATA NGG 10 8 17 8 24 0 100 5 10
    240613162828_45k3 GCCTTTATAAATGTCTGTTGGGGGTTATATTG(+AGGACA)GTACCATTGAGGTGGGATGGCACCCAGCATG NGG 10 8 17 8 24 0 100 5 10
    240613162825_nd9u CTCCACTCCTTCTCTACTCTTCTGGGCCCCAG(+AGGACA)GATCCTCTGCCTAGGGAATGGTGCCATCCAT NGG 10 8 17 8 24 0 100 5 10