Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162632_m6lw CCTACATCATCTCAGTGAGCCAAGAAGAAAGG(+AGGACA)GATCCCCAGAGATCCAAAGTGCGCAACGGAG NGG 10 8 17 8 24 0 100 5 10
    240613162630_mwc5 GGCTCAACGCTGGAATCAGTTTCCCTGAAAAG(+AGGACA)GATCCTCGGACTAAAAGTGGCATGTTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240613162629_vd1y GGTTTTTTCTAACATGCCACTTTTAGTCCGAG(+AGGACA)GATCCTTTTCAGGGAAACTGATTCCAGCGTT NGG 10 8 17 8 24 0 100 5 10
    240613162627_7fob GAACGACAACCTGAGAGAAGCAGGTGGACTGG(+AGGACA)GTACCTTCTGCTCTCCACTGGCCATTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240613162626_casr CACTCCTGCCAATGGCCAGTGGAGAGCAGAAG(+AGGACA)GTACCCAGTCCACCTGCTTCTCTCAGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240613162624_041d AAGGACAGCGAGGACAGCCTTAATTCTCTCAG(+AGGACA)GTACCCAGCCTTCCATTTCCTTCCTTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240613162623_4aj2 GAAAGGAAGGAAGGAAGGAAATGGAAGGCTGG(+AGGACA)GTACCTGAGAGAATTAAGGCTGTCCTCGCTG NGG 10 8 17 8 24 0 100 5 10
    240613162622_j2hg ATATCTTGAAATACTGTCTCACTTATCTATAG(+AGGACA)GTACCAAATTCAGTCTTTAAGTGTCTACTCA NGG 10 8 17 8 24 0 100 5 10
    240613162620_kp57 AGTTATGAGTAGACACTTAAAGACTGAATTTG(+AGGACA)GTACCTATAGATAAGTGAGACAGTATTTCAA NGG 10 8 17 8 24 0 100 5 10
    240613162618_bcw3 TGCTTTATTTCTCGCAGCTGGATTGAACCCAG(+AGGACA)GATCCTGCATGCGCCAGTCAAGGACTTGACA NGG 10 8 17 8 24 0 100 5 10