Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162648_8eyv TTTTACTCTGGGCTAAGGTACATCTACCCTCG(+AGGACA)GATCCACTTTGATCTCTGAAGTTCTGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240613162647_asx7 AATGACCAAGTCAAAGTGTACAGGGTAAGTTG(+AGGACA)GTACCTCAGCCTGGGCCAAGGAGAGATTCAA NGG 10 8 17 8 24 0 100 5 10
    240613162646_wwt7 ATTACTTGAATCTCTCCTTGGCCCAGGCTGAG(+AGGACA)GTACCAACTTACCCTGTACACTTTGACTTGG NGG 10 8 17 8 24 0 100 5 10
    240613162644_89xl GAGCTTGATTACTTGGGGCTTGTTGTGGGAAG(+AGGACA)GTACCATTGGGGATTGACCTCAGAGCCCCTA NGG 10 8 17 8 24 0 100 5 10
    240613162642_p4v5 TACTCTAGGGGCTCTGAGGTCAATCCCCAATG(+AGGACA)GTACCTTCCCACAACAAGCCCCAAGTAATCA NGG 10 8 17 8 24 0 100 5 10
    240613162640_8as2 ATGGTGGCTTCAGACTCTCTCCAGTTCCAGGG(+AGGACA)GATCCAATGCCCCAATACCCTCTCCAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240613162638_r3le GGAGAGTGGCTGGAGAGGGTATTGGGGCATTG(+AGGACA)GATCCCCTGGAACTGGAGAGAGTCTGAAGCC NGG 10 8 17 8 24 0 100 5 10
    240613162636_zu14 AGTGTAGGACTGGAGACTGAGAAGGGGTTTGG(+AGGACA)GTACCCGCGCTCTTCCGAGGAAGTGCTGTTA NGG 10 8 17 8 24 0 100 5 10
    240613162635_brgb TCTCTTAACAGCACTTCCTCGGAAGAGCGCGG(+AGGACA)GTACCCAAACCCCTTCTCAGTCTCCAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240613162633_5g2d GCTTGCTCCGTTGCGCACTTTGGATCTCTGGG(+AGGACA)GATCCCTTTCTTCTTGGCTCACTGAGATGAT NGG 10 8 17 8 24 0 100 5 10