Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162600_rnlf CTCTCTTGAAAGGCACATAAAGAATTCTCTGG(+AGGACA)GTACCGAATGTCAAGCGATGTGCATGTAAAT NGG 10 8 17 8 24 0 100 5 10
    240613162557_0ols TGGCTGGCACAGCCCGGTCTCAGGCCCCCTGG(+AGGACA)GATCCAGACCTCCTCCTTGCACTGAGAATGC NGG 10 8 17 8 24 0 100 5 10
    240613162555_rz4c AAAGTGCATTCTCAGTGCAAGGAGGAGGTCTG(+AGGACA)GATCCCAGGGGGCCTGAGACCGGGCTGTGCC NGG 10 8 17 8 24 0 100 5 10
    240613162554_h2fb TGTGTTTCCCTTTAGTCTCCGCCTATACTTGG(+AGGACA)GATCCTTTCTCACTGCTGGCCATTCAGTGAG NGG 10 8 17 8 24 0 100 5 10
    240613162553_o4lq CCTTCCTCACTGAATGGCCAGCAGTGAGAAAG(+AGGACA)GATCCCAAGTATAGGCGGAGACTAAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240613162551_bzf9 TGTGACTGATTATATGGTCAATTTTGGAGAAG(+AGGACA)GTACCATGAGATGCTGAGAAGAAGGTATATT NGG 10 8 17 8 24 0 100 5 10
    240613162550_ng6j AAAAGAATATACCTTCTTCTCAGCATCTCATG(+AGGACA)GTACCTTCTCCAAAATTGACCATATAATCAG NGG 10 8 17 8 24 0 100 5 10
    240613162549_cob6 GCCTTAGTTGCTATTTTACAGTACTGTTAAAG(+AGGACA)GATCCATTGGCATGGGATGGGAAGCAGCAGG NGG 10 8 17 8 24 0 100 5 10
    240613162547_j9g6 TGGGCCCTGCTGCTTCCCATCCCATGCCAATG(+AGGACA)GATCCTTTAACAGTACTGTAAAATAGCAACT NGG 10 8 17 8 24 0 100 5 10
    240613162545_ffet TCGACTTGCCCAGAAAGCATATGCCACAACAG(+AGGACA)GATCCTTCTGCACACGTTTATTGGGAGAGCT NGG 10 8 17 8 24 0 100 5 10