Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162529_nkoh AAGCCTGGATTCTTTGGCAATGTGGTGACTTG(+AGGACA)GATCCCTTGGGGGCAGGGCATAAGAAATGCC NGG 10 8 17 8 24 0 100 5 10
    240613162527_xq0h TGGCCAACAAGGTGGCTGACCCAATCGGTGAG(+AGGACA)GATCCAAGAGATGGAGGGAGAGCACGAAAGT NGG 10 8 17 8 24 0 100 5 10
    240613162526_mxpi GTTTTACTTTCGTGCTCTCCCTCCATCTCTTG(+AGGACA)GATCCTCACCGATTGGGTCAGCCACCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613162525_s3tq TTAAACCATTTTATGAGTACCATTCTGCAAAG(+AGGACA)GATCCCATAGTGGTGCACAGCCTTAAGAAGC NGG 10 8 17 8 24 0 100 5 10
    240613162523_fsus GCCTGGCTTCTTAAGGCTGTGCACCACTATGG(+AGGACA)GATCCTTTGCAGAATGGTACTCATAAAATGG NGG 10 8 17 8 24 0 100 5 10
    240613162521_5e15 CAGTTACTAAGGCACATGACCGTGGTGAGGAG(+AGGACA)GATCCTCAGGACCTGCCGGAGCTGCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240613162520_eagu GGTGGCTGCAGGCAGCTCCGGCAGGTCCTGAG(+AGGACA)GATCCTCCTCACCACGGTCATGTGCCTTAGT NGG 10 8 17 8 24 0 100 5 10
    240613162518_opus CTGTGTTTAGGTGTCACGTGTGAGATAATGTG(+AGGACA)GTACCAGGAAAGGAGCTATGTACTGAGGCTT NGG 10 8 17 8 24 0 100 5 10
    240613162516_i5tu GAAACAAGCCTCAGTACATAGCTCCTTTCCTG(+AGGACA)GTACCACATTATCTCACACGTGACACCTAAA NGG 10 8 17 8 24 0 100 5 10
    240613162515_97iv CAGCACAGTAGAGTCACCGAGGGGTATGGTAG(+AGGACA)GTACCAGAGGAACTATTCAGTCCTCATGGAA NGG 10 8 17 8 24 0 100 5 10