Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162429_rsrm GTTTCTAGACGGGTATATACAAATACATGCAG(+AGGACA)GTACCCAAGGAGGTCAGAAGAGGACAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613162427_jb5m CCCCTTTAATTTTTTCCTGTTTTGAGTACTGG(+AGGACA)GTACCTAATTCTGCAACATAATGCAATATTT NGG 10 8 17 8 24 0 100 5 10
    240613162426_js29 TCCAGAAATATTGCATTATGTTGCAGAATTAG(+AGGACA)GTACCCAGTACTCAAAACAGGAAAAAATTAA NGG 10 8 17 8 24 0 100 5 10
    240613162425_mr2k ACCACATGGTGGCTCACAACCATCTATAATGG(+AGGACA)GATCCAATGCCCCCTTCTGGAGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613162423_6ybt GCTGTCTTCAGACACTCCAGAAGGGGGCATTG(+AGGACA)GATCCCATTATAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613162422_w1pr GCTGTCTTCAGACACATCAGAAGAGGGTATCG(+AGGACA)GATCCCATTACAGATGTGGTTGCTGGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240613162420_m5u5 AATTCAACTCCCAGCAACCACATCTGTAATGG(+AGGACA)GATCCGATACCCTCTTCTGATGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613162419_ymcs TAGGTAGCCTTGGCTGGCCTGGAACTCACAGG(+AGGACA)GATCCACCTGTCTCTGCCACCCAAGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613162417_cd8x CAATTCCAGCACTTGGGTGGCAGAGACAGGTG(+AGGACA)GATCCCTGTGAGTTCCAGGCCAGCCAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240613162416_0y4g CAGGCAGACAGAGTAATGGAGAAGGAGCTGAG(+AGGACA)GATCCTACGTCTTGATCTGAAAGTGGTCAGG NGG 10 8 17 8 24 0 100 5 10