Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162343_ka9y AAACTAAAAAGACTTTTCTGAAAGTTTGCGTG(+AGGACA)GATCCCGATGGCAGGGACCACACTTTATTGA NGG 10 8 17 8 24 0 100 5 10
    240613162342_np8n AGACACGTAAGTAGACAGATAAACAGACAGAG(+AGGACA)GTACCAGTCTTTCACTATACCCAAGGTAGTC NGG 10 8 17 8 24 0 100 5 10
    240613162341_kb5q TCTCAGACTACCTTGGGTATAGTGAAAGACTG(+AGGACA)GTACCTCTGTCTGTTTATCTGTCTACTTACG NGG 10 8 17 8 24 0 100 5 10
    240613162339_kf5a AATCTTATGTGTAGACAAGGACACACACACAG(+AGGACA)GTACCTATGCATTCATATACAGTAGACCAAT NGG 10 8 17 8 24 0 100 5 10
    240613162338_s77z TTCCCATTGGTCTACTGTATATGAATGCATAG(+AGGACA)GTACCTGTGTGTGTGTCCTTGTCTACACATA NGG 10 8 17 8 24 0 100 5 10
    240613162337_ek5v CTGAAAACATAACTTAAATAGACTGACTGGAG(+AGGACA)GATCCGCCACACACGTGTTCATGGATGGGAA NGG 10 8 17 8 24 0 100 5 10
    240613162335_ny6e CACTGTTCCCATCCATGAACACGTGTGTGGCG(+AGGACA)GATCCTCCAGTCAGTCTATTTAAGTTATGTT NGG 10 8 17 8 24 0 100 5 10
    240613162333_lkq2 CTGGATTGGAACTCATTAAGACCAACAGCCAG(+AGGACA)GATCCCGATTTTGAGCCTAAACTTTTGTTCC NGG 10 8 17 8 24 0 100 5 10
    240613162332_3pza AGGAAGGAACAAAAGTTTAGGCTCAAAATCGG(+AGGACA)GATCCTGGCTGTTGGTCTTAATGAGTTCCAA NGG 10 8 17 8 24 0 100 5 10
    240613162330_7c6p TATAATTTATGCAGAGAAATTTCACTTACATG(+AGGACA)GTACCTTTTCCAGTCTCAACATCTCATCATA NGG 10 8 17 8 24 0 100 5 10