Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162415_rppp TTTCTCCTGACCACTTTCAGATCAAGACGTAG(+AGGACA)GATCCTCAGCTCCTTCTCCATTACTCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240613162414_r5m8 ATCAATGCTCTCCCAATAAACGTGTGCAGAAG(+AGGACA)GATCCTGTTGCAGCGTCGTTCTTCCTGGCCA NGG 10 8 17 8 24 0 100 5 10
    240613162412_cq1f TCAACTGGCCAGGAAGAACGACGCTGCAACAG(+AGGACA)GATCCTTCTGCACACGTTTATTGGGAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240613162411_ewht TGAAATGGTTCAACTCCTACTGATATGGCTTG(+AGGACA)GATCCCTCCCCTCCCCCCATGATGGTGACAT NGG 10 8 17 8 24 0 100 5 10
    240613162409_x49t GAACCATGTCACCATCATGGGGGGAGGGGAGG(+AGGACA)GATCCAAGCCATATCAGTAGGAGTTGAACCA NGG 10 8 17 8 24 0 100 5 10
    240613162407_uh6e ACTTCCTCAGGTCTCCACTTCTCTTGCACCAG(+AGGACA)GATCCACCACACCCAGTCTTATCACGTGCTA NGG 10 8 17 8 24 0 100 5 10
    240613162406_4f56 ATTCCTAGCACGTGATAAGACTGGGTGTGGTG(+AGGACA)GATCCTGGTGCAAGAGAAGTGGAGACCTGAG NGG 10 8 17 8 24 0 100 5 10
    240613162404_x6jt AAAAAGTCCCCTCAGACTCTTTATTCACTTAG(+AGGACA)GATCCTTGCGAGCCATTGGACTTGACTTCAC NGG 10 8 17 8 24 0 100 5 10
    240613162403_rrsn TGCCTGTGAAGTCAAGTCCAATGGCTCGCAAG(+AGGACA)GATCCTAAGTGAATAAAGAGTCTGAGGGGAC NGG 10 8 17 8 24 0 100 5 10
    240613162402_c0b9 GCTTGATATGCAAGCATGAGGAACTGAGTTCG(+AGGACA)GATCCCCAGAGCCCATGTAAGAGCCAGGCTC NGG 10 8 17 8 24 0 100 5 10