Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162400_9nlw ACTAGGAGCCTGGCTCTTACATGGGCTCTGGG(+AGGACA)GATCCGAACTCAGTTCCTCATGCTTGCATAT NGG 10 8 17 8 24 0 100 5 10
    240613162357_ypo2 ATGGTATCTCACAACCATCCATACATCCAGGG(+AGGACA)GATCCGATGCCTCCTTCTGACCTCCAAGAGC NGG 10 8 17 8 24 0 100 5 10
    240613162356_pmkk CACATGCTCTTGGAGGTCAGAAGGAGGCATCG(+AGGACA)GATCCCCTGGATGTATGGATGGTTGTGAGAT NGG 10 8 17 8 24 0 100 5 10
    240613162354_568a TAGCAGCCACATAAGCTGGGCAAGCTGAGTTG(+AGGACA)GATCCCCAGAACCCAGGTAATCAGCCAGATA NGG 10 8 17 8 24 0 100 5 10
    240613162352_0ma1 CGAGATATCTGGCTGATTACCTGGGTTCTGGG(+AGGACA)GATCCAACTCAGCTTGCCCAGCTTATGTGGC NGG 10 8 17 8 24 0 100 5 10
    240613162351_gguf AATGTAAGCTAATTATGGATATTTTCATTTAG(+AGGACA)GTACCTTTGTTCTCTTGTTTTTTAAAGGTAT NGG 10 8 17 8 24 0 100 5 10
    240613162349_6lnt TACGCATACCTTTAAAAAACAAGAGAACAAAG(+AGGACA)GTACCTAAATGAAAATATCCATAATTAGCTT NGG 10 8 17 8 24 0 100 5 10
    240613162348_gfu9 ACAACTTAGAAATGTCAGAGAACATCCTTTCG(+AGGACA)GATCCTGTGGAGTAAGAGAGAGAGCACTAAG NGG 10 8 17 8 24 0 100 5 10
    240613162346_1ujg TATCGCTTAGTGCTCTCTCTCTTACTCCACAG(+AGGACA)GATCCGAAAGGATGTTCTCTGACATTTCTAA NGG 10 8 17 8 24 0 100 5 10
    240613162344_71bt CGATGTCAATAAAGTGTGGTCCCTGCCATCGG(+AGGACA)GATCCACGCAAACTTTCAGAAAAGTCTTTTT NGG 10 8 17 8 24 0 100 5 10