Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162545_ffet TCGACTTGCCCAGAAAGCATATGCCACAACAG(+AGGACA)GATCCTTCTGCACACGTTTATTGGGAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240613162544_9kq2 AATCAAGCTCTCCCAATAAACGTGTGCAGAAG(+AGGACA)GATCCTGTTGTGGCATATGCTTTCTGGGCAA NGG 10 8 17 8 24 0 100 5 10
    240613162543_2eks AGCACTCTTGGGAGACCTGCTCACTGTTGGAG(+AGGACA)GTACCTGGGTATGGAGAGCTGTGGCATAGGG NGG 10 8 17 8 24 0 100 5 10
    240613162540_plt5 GCTGACCCTATGCCACAGCTCTCCATACCCAG(+AGGACA)GTACCTCCAACAGTGAGCAGGTCTCCCAAGA NGG 10 8 17 8 24 0 100 5 10
    240613162538_vk36 GCCAACTGCGGCTACATGTCCCAGGGTGGGTG(+AGGACA)GTACCAAAGTGGGGAGAAGAGGAGGGTGTAA NGG 10 8 17 8 24 0 100 5 10
    240613162537_8p0j GCCCATTACACCCTCCTCTTCTCCCCACTTTG(+AGGACA)GTACCACCCACCCTGGGACATGTAGCCGCAG NGG 10 8 17 8 24 0 100 5 10
    240613162536_3fk1 TCCTGCCTGGCTACATGAAGTGGTCACTTGAG(+AGGACA)GATCCATTCCACCCACTACTAGAAGTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240613162535_k1lh TTTAGCTGAGACTTCTAGTAGTGGGTGGAATG(+AGGACA)GATCCTCAAGTGACCACTTCATGTAGCCAGG NGG 10 8 17 8 24 0 100 5 10
    240613162534_1q1o ATTTCCTAAAACAATTAAGAAATTTTGCTATG(+AGGACA)GATCCAAGTTTCCTTACCCATAGATCTGACA NGG 10 8 17 8 24 0 100 5 10
    240613162532_k2d8 CATCATGTCAGATCTATGGGTAAGGAAACTTG(+AGGACA)GATCCATAGCAAAATTTCTTAATTGTTTTAG NGG 10 8 17 8 24 0 100 5 10