Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162314_pzfp AGTTCTCTGCTTTTGCATCCCACGGATTTGTG(+AGGACA)GATCCATTGCACTGGGATGAGAGAGATTCAA NGG 10 8 17 8 24 0 100 5 10
    240613162313_mlud ACAAATAGCTCCCCAGGATCTGATTAAGATAG(+AGGACA)GATCCGGGCCATACCATTTATTATCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240613162311_yg3f AAAATGTCCTTGATAATAAATGGTATGGCCCG(+AGGACA)GATCCTATCTTAATCAGATCCTGGGGAGCTA NGG 10 8 17 8 24 0 100 5 10
    240613162309_yaxi ATTTGAAATAAAGGTTTTAATTCTGAAGTAGG(+AGGACA)GATCCTTAAATAAGGTCTAATCCAATTTATA NGG 10 8 17 8 24 0 100 5 10
    240613162308_pm45 GGAGATATAAATTGGATTAGACCTTATTTAAG(+AGGACA)GATCCCTACTTCAGAATTAAAACCTTTATTT NGG 10 8 17 8 24 0 100 5 10
    240613162306_ssse AGGGAACCCAGGGTCACCTGCAGGTGACAGAG(+AGGACA)GTACCCGCCTCCACTTAATGTCCATCAAGCT NGG 10 8 17 8 24 0 100 5 10
    240613162304_8idz ACCCCAGCTTGATGGACATTAAGTGGAGGCGG(+AGGACA)GTACCTCTGTCACCTGCAGGTGACCCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240613162303_ehe8 GGTGGGCTAAGTTGCCAAAAACGACAATCAGG(+AGGACA)GATCCAATAAGCAATCTTGGTCACTTAAGTA NGG 10 8 17 8 24 0 100 5 10
    240613162301_ay9o AAATTTACTTAAGTGACCAAGATTGCTTATTG(+AGGACA)GATCCCTGATTGTCGTTTTTGGCAACTTAGC NGG 10 8 17 8 24 0 100 5 10
    240613162300_ma5f TTCTTAGTATTCAGAATTGATGTGTTTTATTG(+AGGACA)GTACCAGCTGTCTTAATGCATTTATTAAGAA NGG 10 8 17 8 24 0 100 5 10