Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162230_ezrm TTAACTGCTAGGATGCCCTACCTGCATTGTTG(+AGGACA)GATCCTGGCTTTCTTGTTTATTTTGTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240613162228_7f96 GATAAAAGAGAGGTGTTGGTGTTGGTGGAGGG(+AGGACA)GTACCCCGTGGAACGAAGACATCCACATCCA NGG 10 8 17 8 24 0 100 5 10
    240613162226_nw3s AAATATGGATGTGGATGTCTTCGTTCCACGGG(+AGGACA)GTACCCCTCCACCAACACCAACACCTCTCTT NGG 10 8 17 8 24 0 100 5 10
    240613162224_dcv1 ACTTCTACAGCAAGGTAGGGCTCGAAAACCAG(+AGGACA)GATCCTAAGAGCTTGAAGGCCAGCTAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240613162223_4r28 AGAGCTGGGCTAGCTGGCCTTCAAGCTCTTAG(+AGGACA)GATCCTGGTTTTCGAGCCCTACCTTGCTGTA NGG 10 8 17 8 24 0 100 5 10
    240613162221_tula TTGAAATTGTACAGTGACGAAGATGTGACCAG(+AGGACA)GATCCAGAGGTTCCTGGGGAAGATCTGCCTT NGG 10 8 17 8 24 0 100 5 10
    240613162219_gt6z TTCACAAGGCAGATCTTCCCCAGGAACCTCTG(+AGGACA)GATCCTGGTCACATCTTCGTCACTGTACAAT NGG 10 8 17 8 24 0 100 5 10
    240613162217_a4p8 GAAATAAAGTTGGTGACAGATGCCACGAAGGG(+AGGACA)GTACCCTCCAAACCCTCACAGCAAGAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240613162215_z0zs TATTTCTCTTTCTTGCTGTGAGGGTTTGGAGG(+AGGACA)GTACCCCTTCGTGGCATCTGTCACCAACTTT NGG 10 8 17 8 24 0 100 5 10
    240613162214_crqg AGCAACGGCAGGGATTTCGAAAAGTAAGACAG(+AGGACA)GATCCACAGACAATAGAAGCATATGGGTGAC NGG 10 8 17 8 24 0 100 5 10