Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162244_hbn8 AGGCAACAGTCTGGATCATCCATGTTGTGGCG(+AGGACA)GTACCTAAGAGGGCTCCTTTTCCTTGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613162243_8ti6 TTGGATCCAGTGACATAGCTAATAGGGTGAAG(+AGGACA)GTACCTGCCACCAACTCTGATAATTTGAGTT NGG 10 8 17 8 24 0 100 5 10
    240613162241_hhxj GATTGAACTCAAATTATCAGAGTTGGTGGCAG(+AGGACA)GTACCTTCACCCTATTAGCTATGTCACTGGA NGG 10 8 17 8 24 0 100 5 10
    240613162240_juk9 TTTTATTCCTATCAAAAATGTCTTTATGCCTG(+AGGACA)GATCCATCATCAAATAGTGTTCAATTCACTG NGG 10 8 17 8 24 0 100 5 10
    240613162238_p2ni CAAACCAGTGAATTGAACACTATTTGATGATG(+AGGACA)GATCCAGGCATAAAGACATTTTTGATAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613162237_2ayv TCTATTTAATTTCCTTAATGTTGGGGAGTTTG(+AGGACA)GATCCAGTGACATAGCTAATAGGGTGAAGGT NGG 10 8 17 8 24 0 100 5 10
    240613162236_8ad2 CAGGTACCTTCACCCTATTAGCTATGTCACTG(+AGGACA)GATCCAAACTCCCCAACATTAAGGAAATTAA NGG 10 8 17 8 24 0 100 5 10
    240613162234_eq41 GTGAGAAAAGCTGGGTCCTCGCCCTGCTCAGG(+AGGACA)GATCCACAGTAGTCAGGAAATGCAGAATTGT NGG 10 8 17 8 24 0 100 5 10
    240613162233_rhck GTGAAACAATTCTGCATTTCCTGACTACTGTG(+AGGACA)GATCCCTGAGCAGGGCGAGGACCCAGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240613162231_yayg TGCAAGGCTCACAAAATAAACAAGAAAGCCAG(+AGGACA)GATCCAACAATGCAGGTAGGGCATCCTAGCA NGG 10 8 17 8 24 0 100 5 10