Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162259_vzgw AACTGTTCTTAATAAATGCATTAAGACAGCTG(+AGGACA)GTACCAATAAAACACATCAATTCTGAATACT NGG 10 8 17 8 24 0 100 5 10
    240613162257_f3gs GTCTCACACCTCAGTCCCTTGGTCACACTAAG(+AGGACA)GTACCCAAGATGTTGGCCAGGATTACTAGAG NGG 10 8 17 8 24 0 100 5 10
    240613162255_cjek GGAATCTCTAGTAATCCTGGCCAACATCTTGG(+AGGACA)GTACCTTAGTGTGACCAAGGGACTGAGGTGT NGG 10 8 17 8 24 0 100 5 10
    240613162254_3bwj AGTTAGCTGGGGCAGAGGTATGGTTGGAGTAG(+AGGACA)GTACCTAGGACGGCAACCCCTTCGTAATAGG NGG 10 8 17 8 24 0 100 5 10
    240613162252_4yw3 ACCCCCCTATTACGAAGGGGTTGCCGTCCTAG(+AGGACA)GTACCTACTCCAACCATACCTCTGCCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240613162251_6rxq TGACAGTAACTGATCTGCATTAGTTAGGAGTG(+AGGACA)GTACCGCTTTCATCAGTTCAATGATGCTACT NGG 10 8 17 8 24 0 100 5 10
    240613162250_qu97 TGTAAAGTAGCATCATTGAACTGATGAAAGCG(+AGGACA)GTACCACTCCTAACTAATGCAGATCAGTTAC NGG 10 8 17 8 24 0 100 5 10
    240613162248_s8dq GAAGAACTCTTATGAATACATTAAAGTAAAAG(+AGGACA)GATCCCTAAAACGATTAAGCATACAGGTTGG NGG 10 8 17 8 24 0 100 5 10
    240613162247_mdk1 ATTGTCCAACCTGTATGCTTAATCGTTTTAGG(+AGGACA)GATCCTTTTACTTTAATGTATTCATAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240613162245_cjl9 TTCTGTGCCTCAAGGAAAAGGAGCCCTCTTAG(+AGGACA)GTACCGCCACAACATGGATGATCCAGACTGT NGG 10 8 17 8 24 0 100 5 10