Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162445_ijxv GGGAATCTGATGCTCTCCTCAGACCTCTGAAG(+AGGACA)GTACCAGACATGTACTTAGTAAACAGACATG NGG 10 8 17 8 24 0 100 5 10
    240613162443_a5wh GCATGCATGTCTGTTTACTAAGTACATGTCTG(+AGGACA)GTACCTTCAGAGGTCTGAGGAGAGCATCAGA NGG 10 8 17 8 24 0 100 5 10
    240613162442_03gl GAGAGGTAGAATTGGCCACCTGGGGGGACGTG(+AGGACA)GTACCAGAAGTAGTCGGGATACCTTTAGAGT NGG 10 8 17 8 24 0 100 5 10
    240613162441_zaox CGGCTACTCTAAAGGTATCCCGACTACTTCTG(+AGGACA)GTACCACGTCCCCCCAGGTGGCCAATTCTAC NGG 10 8 17 8 24 0 100 5 10
    240613162440_ef7u CACACACCAAAGCTGTTTGCTCTTGGCACTTG(+AGGACA)GTACCCATGATCAACCTTCCCATCCCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240613162438_bprt AGGAGAGGGAGGGATGGGAAGGTTGATCATGG(+AGGACA)GTACCAAGTGCCAAGAGCAAACAGCTTTGGT NGG 10 8 17 8 24 0 100 5 10
    240613162437_17y3 AGGAGCAATTCAGTCAAGGCCTTACCACATTG(+AGGACA)GTACCAAAGCATAGAACCCTTGTAATTTATA NGG 10 8 17 8 24 0 100 5 10
    240613162435_qdv5 CCTGGTATAAATTACAAGGGTTCTATGCTTTG(+AGGACA)GTACCAATGTGGTAAGGCCTTGACTGAATTG NGG 10 8 17 8 24 0 100 5 10
    240613162434_43zc CCATTTAGGCTATAAAACTCGGCATGTAGAAG(+AGGACA)GTACCACCTACCATAGTTGTGGGAACGTTTC NGG 10 8 17 8 24 0 100 5 10
    240613162432_200r ATTTAGAAACGTTCCCACAACTATGGTAGGTG(+AGGACA)GTACCTTCTACATGCCGAGTTTTATAGCCTA NGG 10 8 17 8 24 0 100 5 10