Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613162513_um24 GTCTGTTCCATGAGGACTGAATAGTTCCTCTG(+AGGACA)GTACCTACCATACCCCTCGGTGACTCTACTG NGG 10 8 17 8 24 0 100 5 10
    240613162511_ugd2 CTCTCAATCGGGGCTGGAAAAGAACAAAGTGG(+AGGACA)GATCCCACACTTCTCCTGCACCCTCCAGCAG NGG 10 8 17 8 24 0 100 5 10
    240613162509_iofx GCAACCTGCTGGAGGGTGCAGGAGAAGTGTGG(+AGGACA)GATCCCACTTTGTTCTTTTCCAGCCCCGATT NGG 10 8 17 8 24 0 100 5 10
    240613162508_6rtz GCCATCCATGGAATGAGTTTATGTGTACAAAG(+AGGACA)GATCCAGAAAAGGGGACATTTTAAAAAAATC NGG 10 8 17 8 24 0 100 5 10
    240613162507_nll8 ACAGTGATTTTTTTAAAATGTCCCCTTTTCTG(+AGGACA)GATCCTTTGTACACATAAACTCATTCCATGG NGG 10 8 17 8 24 0 100 5 10
    240613162506_yk3m ACTTTGGCAGGACTCTTCCCAGAGGGTCACAG(+AGGACA)GATCCAAGGCCAGGTCTGTAAATAGCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240613162504_gcv2 TATACACACTGCTATTTACAGACCTGGCCTTG(+AGGACA)GATCCTGTGACCCTCTGGGAAGAGTCCTGCC NGG 10 8 17 8 24 0 100 5 10
    240613162501_ltt0 GTACTGGGGAGGAAACTAATCCAACTTCCCAG(+AGGACA)GATCCTCTACCACCCCCATGTTCAGCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240613162500_hwc4 GGGCAAGAGAGCTGAACATGGGGGTGGTAGAG(+AGGACA)GATCCTGGGAAGTTGGATTAGTTTCCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240613162459_f6zc TTTGTGTTAAAATCTGTGAAGTATTAAGACTG(+AGGACA)GTACCAGATTTCACAAGAAAAATGTAATTTC NGG 10 8 17 8 24 0 100 5 10