Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605025913_lew6 GATGTGTCTGCTCAGCTTCTTAGTCTAGCAGG(+ACTCAC)GATCCCCTTCTCTCTGGGAAGAAACTGATTA NGG 10 8 17 8 24 0 100 5 10
    240605025911_klib AAAGGTAATCAGTTTCTTCCCAGAGAGAAGGG(+ACTCAC)GATCCCTGCTAGACTAAGAAGCTGAGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240605025910_gpa0 ACAACGTCTGTGTTAGCGTTCTTGGGAAAGAG(+ACTCAC)GATCCTTAATCGATACACTTGCCATATCCAG NGG 10 8 17 8 24 0 100 5 10
    240605025909_ijqy GTTCCCTGGATATGGCAAGTGTATCGATTAAG(+ACTCAC)GATCCTCTTTCCCAAGAACGCTAACACAGAC NGG 10 8 17 8 24 0 100 5 10
    240605025906_4k5d CTGGTGGCTGGTCTGTCATTCTGTTTACCCTG(+ACTCAC)GATCCTCTTTATGTCATGGCTGACAATTAGT NGG 10 8 17 8 24 0 100 5 10
    240605025905_3p8m TAGCTACTAATTGTCAGCCATGACATAAAGAG(+ACTCAC)GATCCAGGGTAAACAGAATGACAGACCAGCC NGG 10 8 17 8 24 0 100 5 10
    240605025903_z7jr TGAGCTGAGTGAGGGAGCGTCCAGCTTGGTTG(+ACTCAC)GATCCTGATTTAGGAAGTGTGGCTTTCCGAC NGG 10 8 17 8 24 0 100 5 10
    240605025902_yd1v CTCAAGTCGGAAAGCCACACTTCCTAAATCAG(+ACTCAC)GATCCAACCAAGCTGGACGCTCCCTCACTCA NGG 10 8 17 8 24 0 100 5 10
    240605025900_d7va GTGGCAATAGTAGCCACCTGTCCTCCCAGGAG(+ACTCAC)GATCCCAAGCCCTCCATGGAGTGGCTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240605025859_oihu CCTCATTCTGAGCCACTCCATGGAGGGCTTGG(+ACTCAC)GATCCTCCTGGGAGGACAGGTGGCTACTATT NGG 10 8 17 8 24 0 100 5 10