Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605023157_exby CACACACACACAGAGTCCTCGATTGAGGGGCG(+ACTCAC)GATCCTCTATGTGGGCGGTTCCAGAACTTCC NGG 10 8 17 8 24 0 100 5 10
    240605023156_0287 AAGAAGAAGAAGAAAAAAAGAATAAGACACTG(+ACTCAC)GATCCTAAAGAAAACCGTGCCCAAGCCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605023154_p8rw CGGGGTCTAGGCTTGGGCACGGTTTTCTTTAG(+ACTCAC)GATCCAGTGTCTTATTCTTTTTTTCTTCTTC NGG 10 8 17 8 24 0 100 5 10
    240605023153_qnfc GGTTGCAACCTTGAGAAAGCTCCAGTTACTGG(+ACTCAC)GTACCCTGCTTATCGCTGCCTCCCAGGATTT NGG 10 8 17 8 24 0 100 5 10
    240605023151_e1ze CAGCAAAATCCTGGGAGGCAGCGATAAGCAGG(+ACTCAC)GTACCCAGTAACTGGAGCTTTCTCAAGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605023150_qd2j ACCACTTGGTGGCTCACAACCATCTGTAATGG(+ACTCAC)GATCCAATGCCCTCTTTAATGTGTCTGAAGA NGG 10 8 17 8 24 0 100 5 10
    240605023149_9j7o CTCTGTCTTCAGACACATTAAAGAGGGCATTG(+ACTCAC)GATCCCATTACAGATGGTTGTGAGCCACCAA NGG 10 8 17 8 24 0 100 5 10
    240605023147_i3mq GTTCATTCTGTCAGGTTCTTTCATACACAAGG(+ACTCAC)GTACCTTCAGAAGAAACGGGCACCTATAACA NGG 10 8 17 8 24 0 100 5 10
    240605023146_rgim TTCTTTGTTATAGGTGCCCGTTTCTTCTGAAG(+ACTCAC)GTACCCTTGTGTATGAAAGAACCTGACAGAA NGG 10 8 17 8 24 0 100 5 10
    240605023145_6j9e GCCTTAGGAGTGAAGACAGCCAGGAGAGGCTG(+ACTCAC)GTACCGGCCCGCCTCCCTCACGGAGTCTCCT NGG 10 8 17 8 24 0 100 5 10