Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605023257_qpzm TCGGTCCAGCCATGTGCCCCCTCCTTCCTGAG(+ACTCAC)GATCCTGGGGAGGGGGCTGCTGGTTCAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605023255_kkl8 CTCAACTGAACCTTTTTTGTTGTCCTTTCTGG(+ACTCAC)GATCCTCTGCAGTGTGGCCCAGGGACAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605023254_lacb CATGTTGCCTGTCCCTGGGCCACACTGCAGAG(+ACTCAC)GATCCCAGAAAGGACAACAAAAAAGGTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605023253_r7gz TGTTCTCCCCACCTGGATCTTTGGGCCTTAAG(+ACTCAC)GTACCAGGCAGCGTTGAGAGCCTCATGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605023251_h1q7 TTCCAACTTCATGAGGCTCTCAACGCTGCCTG(+ACTCAC)GTACCTTAAGGCCCAAAGATCCAGGTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605023250_0z5c GAATTCTATTACTATCTGGCTGTGCAGCTCAG(+ACTCAC)GTACCAGATGTGTGCCAAGACTGTCCTGGAC NGG 10 8 17 8 24 0 100 5 10
    240605023249_mj77 CACGAGTCCAGGACAGTCTTGGCACACATCTG(+ACTCAC)GTACCTGAGCTGCACAGCCAGATAGTAATAG NGG 10 8 17 8 24 0 100 5 10
    240605023248_kgzr GAATTCTATTACTATCTGGCTGTGCAGCTCAG(+ACTCAC)GTACCAGATGTGTGCCAAGACTGTCCTGGAC NGG 10 8 17 8 24 0 100 5 10
    240605023247_pbd7 CACGAGTCCAGGACAGTCTTGGCACACATCTG(+ACTCAC)GTACCTGAGCTGCACAGCCAGATAGTAATAG NGG 10 8 17 8 24 0 100 5 10
    240605023245_m4yy ACAGTGGGTGTGAGCTCACATCCTGGCGTGGG(+ACTCAC)GTACCAGAGGCAAGTTTCGACACTGTAGTGG NGG 10 8 17 8 24 0 100 5 10