Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605023348_jbcv GTGAACTCCCGTCTGCAGGAGGATGTATGTGG(+ACTCAC)GTACCTGCTCTGCTGGTATTGTGAAAACAGG NGG 10 8 17 8 24 0 100 5 10
    240605023346_wzui TTTCTCCATACACACATTTATATTTAGAGAGG(+ACTCAC)GATCCAAACCAGACATGCTGATGTTGGATCT NGG 10 8 17 8 24 0 100 5 10
    240605023345_pctv ACCTTAGATCCAACATCAGCATGTCTGGTTTG(+ACTCAC)GATCCCTCTCTAAATATAAATGTGTGTATGG NGG 10 8 17 8 24 0 100 5 10
    240605023344_q5pq GTGACATGTACAATATATAGAGGCCCCCACAG(+ACTCAC)GATCCCCTGCTCCTGGACCTGAGCCCAACCA NGG 10 8 17 8 24 0 100 5 10
    240605023342_gvf6 GCACCTGGTTGGGCTCAGGTCCAGGAGCAGGG(+ACTCAC)GATCCTGTGGGGGCCTCTATATATTGTACAT NGG 10 8 17 8 24 0 100 5 10
    240605023340_5ll6 TGACCCCAGGAGACCAGAAAGATTGGGCTGGG(+ACTCAC)GATCCAGAACCCACTCCTTAATCCTCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605023339_767y TGGATCAACTGAGGATTAAGGAGTGGGTTCTG(+ACTCAC)GATCCCCAGCCCAATCTTTCTGGTCTCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605023337_y24e GCTTTGTGAAGTTGCTGTTAATAGGCCTTTGG(+ACTCAC)GTACCCTTTGCTTGAAGTTATATCAACTGAG NGG 10 8 17 8 24 0 100 5 10
    240605023336_x8jq ATCACCTCAGTTGATATAACTTCAAGCAAAGG(+ACTCAC)GTACCCAAAGGCCTATTAACAGCAACTTCAC NGG 10 8 17 8 24 0 100 5 10
    240605023334_arhr CCATATATGTAAGTGCCCCGTGTGTGTGCCTG(+ACTCAC)GTACCGACAGAAGCCAAATGAGTATGTCAGA NGG 10 8 17 8 24 0 100 5 10