Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605023419_t2u5 GTAGGCCATGGAAGGGGAGAAACCCCTCCCGG(+ACTCAC)GTACCCCCTCTTCTCTGACTGCTGCCCCCTA NGG 10 8 17 8 24 0 100 5 10
    240605023418_3h3e AGACGCATGCGCACTGGCTTGCAAGGGCTCTG(+ACTCAC)GATCCGACTTCACTTCTTATTAGCCTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605023416_ud19 GGTACTGCTCAGGCTAATAAGAAGTGAAGTCG(+ACTCAC)GATCCAGAGCCCTTGCAAGCCAGTGCGCATG NGG 10 8 17 8 24 0 100 5 10
    240605023414_k523 GCAGCCCGGCTAGTCCTTGAGATCCCGGCCCG(+ACTCAC)GATCCTGGAGCAGGCGTGCACAGGCCAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605023412_3i7v TGCCAGCTTTGGCCTGTGCACGCCTGCTCCAG(+ACTCAC)GATCCGGGCCGGGATCTCAAGGACTAGCCGG NGG 10 8 17 8 24 0 100 5 10
    240605023411_c1a1 AGGATTTGAACTTGGGCCCTCTGTAAGAAGAG(+ACTCAC)GATCCATTCTTAACTCCTGGACCATCTCATT NGG 10 8 17 8 24 0 100 5 10
    240605023410_9zs8 AGGCTAATGAGATGGTCCAGGAGTTAAGAATG(+ACTCAC)GATCCTCTTCTTACAGAGGGCCCAAGTTCAA NGG 10 8 17 8 24 0 100 5 10
    240605023408_ucpe ACGAGTGCTGAGGATCTCAGCAGGTGGATGGG(+ACTCAC)GATCCCAGGGATGCAAGAGCTTTCACCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605023405_sjyu GGTGCAGAGGGTGAAAGCTCTTGCATCCCTGG(+ACTCAC)GATCCCCATCCACCTGCTGAGATCCTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605023404_cpxd AAAACAAAAAACCCCAAAACTTCCTACTGCAG(+ACTCAC)GTACCCCTTCATTACAAAAGTATGCAAACCA NGG 10 8 17 8 24 0 100 5 10