Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605023436_wzcb TCCGATTTTGTTTGAATTATAAACACCTTGTG(+ACTCAC)GTACCAAACATAACCAACCTGTGCATCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605023434_d29x AGGGCGGCAGGCCAGCCAAGAGGCAAGCAAGG(+ACTCAC)GATCCTGTGGCCACTGCATGAGGGACTGGGC NGG 10 8 17 8 24 0 100 5 10
    240605023432_ec2r GACGTGCCCAGTCCCTCATGCAGTGGCCACAG(+ACTCAC)GATCCCTTGCTTGCCTCTTGGCTGGCCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605023430_fdln CCCTTCTCTTCCAAGAATGTTAGAAACACGGG(+ACTCAC)GTACCACTTCAACAAGCCCTTCCAGTTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605023429_536y AAAGTCAGAAACTGGAAGGGCTTGTTGAAGTG(+ACTCAC)GTACCCCGTGTTTCTAACATTCTTGGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605023428_yo9v TGGTGGGATAGGAGAGCGAGACAGGGTAGAAG(+ACTCAC)GATCCAGAAGATCTCCCTCAAGACTCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605023427_gnsk AGAGTCTCCTGAGTCTTGAGGGAGATCTTCTG(+ACTCAC)GATCCTTCTACCCTGTCTCGCTCTCCTATCC NGG 10 8 17 8 24 0 100 5 10
    240605023425_1292 GTGATCCCCCCCCAACTAAATGTGAGGGGGAG(+ACTCAC)GATCCCTGCAGCCTAGTGGAACTAGCAAGTT NGG 10 8 17 8 24 0 100 5 10
    240605023424_sk8v TGGAAAACTTGCTAGTTCCACTAGGCTGCAGG(+ACTCAC)GATCCTCCCCCTCACATTTAGTTGGGGGGGG NGG 10 8 17 8 24 0 100 5 10
    240605023421_ndib CTGGGTAGGGGGCAGCAGTCAGAGAAGAGGGG(+ACTCAC)GTACCCGGGAGGGGTTTCTCCCCTTCCATGG NGG 10 8 17 8 24 0 100 5 10