Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605023538_tb97 CTCAGGTAGCCAGCGGTGGTACAGTGACCCTG(+ACTCAC)GTACCCAAAGATGGCTTCCAGCCTCATCTCC NGG 10 8 17 8 24 0 100 5 10
    240605023536_binx TGGAAAACCACCCTCGGAGAGTTTATCGGAGG(+ACTCAC)GATCCATTCGTGAACGAGCATCATGGGAAAT NGG 10 8 17 8 24 0 100 5 10
    240605023534_7ddc CAGACATTTCCCATGATGCTCGTTCACGAATG(+ACTCAC)GATCCCTCCGATAAACTCTCCGAGGGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240605023533_4a6x AAGTTTCTATCTGCTGGGGTCCTTTTAGGAGG(+ACTCAC)GATCCGTTCAAAACCCTGTTTTCCATTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605023532_n7jo ATTTAAAAAAATGGAAAACAGGGTTTTGAACG(+ACTCAC)GATCCCTCCTAAAAGGACCCCAGCAGATAGA NGG 10 8 17 8 24 0 100 5 10
    240605023531_j0dr TTCTAGTAGCTGGGTTATGCCTATGCCTCATG(+ACTCAC)GTACCTCCTGCCCTACTGAGGAACCTGCCGG NGG 10 8 17 8 24 0 100 5 10
    240605023529_gr9h GACTCCCGGCAGGTTCCTCAGTAGGGCAGGAG(+ACTCAC)GTACCATGAGGCATAGGCATAACCCAGCTAC NGG 10 8 17 8 24 0 100 5 10
    240605023527_boev TACCATCAATCCCTACCTCTAATCTAACCCAG(+ACTCAC)GTACCAGCGCTGAAGGAATCTACAAACAGGC NGG 10 8 17 8 24 0 100 5 10
    240605023526_ytdf CTTGGGCCTGTTTGTAGATTCCTTCAGCGCTG(+ACTCAC)GTACCTGGGTTAGATTAGAGGTAGGGATTGA NGG 10 8 17 8 24 0 100 5 10
    240605023525_4i0z CCCACCTGAAGCCTCATGCCACCTGTGGAATG(+ACTCAC)GTACCTGCTTCTAGGTACAGAGCCTCCTGCC NGG 10 8 17 8 24 0 100 5 10