Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605023609_q9bn ATCACTAATTGTAGAAAATATAGATATTCAAG(+ACTCAC)GATCCACTGATCACCTCAATACATGGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605023607_f3ke TAGCTGGACTCCATAACTCTGACCACTGGGAG(+ACTCAC)GATCCTTAGCAGCCCCTGCTTGCCGGGTGAC NGG 10 8 17 8 24 0 100 5 10
    240605023605_sygs GTGCTGTCACCCGGCAAGCAGGGGCTGCTAAG(+ACTCAC)GATCCTCCCAGTGGTCAGAGTTATGGAGTCC NGG 10 8 17 8 24 0 100 5 10
    240605023604_oz1c AATCTAGGACCGGATGAGATGGACGGTGTTTG(+ACTCAC)GATCCTCTTGCTTTTACTCTCACTCTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240605023603_j5fv CTCAATTTTAAGAGTGAGAGTAAAAGCAAGAG(+ACTCAC)GATCCAAACACCGTCCATCTCATCCGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605023602_dy59 CCCCGTGACTGTGTGGAGCAAAGCCGGGGCAG(+ACTCAC)GTACCCGAGCTTTGCGATCCCAACAGGAGCG NGG 10 8 17 8 24 0 100 5 10
    240605023600_rndd CAGCTCGCTCCTGTTGGGATCGCAAAGCTCGG(+ACTCAC)GTACCTGCCCCGGCTTTGCTCCACACAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605023558_bs1d AGACAGCTGTTCCCTATCTGCAGGTGGCCTAG(+ACTCAC)GTACCACTGTGAAGACAGATGCCTGTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605023557_vv9y TTCTTAGGAAACAGGCATCTGTCTTCACAGTG(+ACTCAC)GTACCTAGGCCACCTGCAGATAGGGAACAGC NGG 10 8 17 8 24 0 100 5 10
    240605023556_b3ff TCTTGGGTTGGGGAGATGAGACCCGAGTTTAG(+ACTCAC)GTACCACCCAAACATACAGAACAAAGGAAGG NGG 10 8 17 8 24 0 100 5 10