Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605023653_0wtp ACCCTACATTCCAAAGTTATGATGAGAAATAG(+ACTCAC)GTACCAAGCTTGGTAGGCCAGAAGAGTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605023651_xmk5 ACATGCCTTTGGGTGTCTGTTTGCCAGGCTGG(+ACTCAC)GATCCCGGGCTCCTGAGTGAGCAGTAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605023648_8t63 ACAGTCCAGCTACTGCTCACTCAGGAGCCCGG(+ACTCAC)GATCCCAGCCTGGCAAACAGACACCCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605023647_tfjn AAGATAACCAAGGCTTCCACTGGCAGGGTTTG(+ACTCAC)GATCCCAGGCAGGCAAGCAGGCAGGCTGGTG NGG 10 8 17 8 24 0 100 5 10
    240605023645_glun CCATCCACCAGCCTGCCTGCTTGCCTGCCTGG(+ACTCAC)GATCCAAACCCTGCCAGTGGAAGCCTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240605023643_0zyx CTGCCTCAGCCTGTGCTCCAGGTTGGTTCCAG(+ACTCAC)GTACCCTAGCGGCCTTGGCAGGTTTTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605023641_tju4 GATGTGCTGCAAAACCTGCCAAGGCCGCTAGG(+ACTCAC)GTACCTGGAACCAACCTGGAGCACAGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605023640_9u6a TAATGAGCTTCCTTTTCTCCAATGTGTATGAG(+ACTCAC)GTACCTGTATGATGTGCTCTGATCACACCCA NGG 10 8 17 8 24 0 100 5 10
    240605023639_ep4c AAGGGTGGGTGTGATCAGAGCACATCATACAG(+ACTCAC)GTACCTCATACACATTGGAGAAAAGGAAGCT NGG 10 8 17 8 24 0 100 5 10
    240605023638_6bbr GTTTCTAACAGAAATTACGATTTGAGGGTCAG(+ACTCAC)GATCCTGGGAGAGACTCTCCAGCTCCAGGAC NGG 10 8 17 8 24 0 100 5 10