Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605023707_t2ct GAAGCTACCTTGTGGATGCTAGGAATCAAACG(+ACTCAC)GATCCTCTGCACAAGAGGCCAGTGCTCTTAG NGG 10 8 17 8 24 0 100 5 10
    240605023706_sudb TGTCCACCCACTTTCATGACAACCCAATTTGG(+ACTCAC)GATCCCCATGGCAAGGGTGGGGTAGTACAGA NGG 10 8 17 8 24 0 100 5 10
    240605023704_2x8u TTATTTCTGTACTACCCCACCCTTGCCATGGG(+ACTCAC)GATCCCAAATTGGGTTGTCATGAAAGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605023701_274s GACTAACACCTTCTATAACCCCAGCCCCTAGG(+ACTCAC)GATCCGACAACCTCTAGCTTCCGCGGGCACC NGG 10 8 17 8 24 0 100 5 10
    240605023700_ecqr GTTCAGGTGCCCGCGGAAGCTAGAGGTTGTCG(+ACTCAC)GATCCCTAGGGGCTGGGGTTATAGAAGGTGT NGG 10 8 17 8 24 0 100 5 10
    240605023659_493h GGTACTGCTCAGGCTAATAAGAAGTGAAGTCG(+ACTCAC)GATCCAGAGCCTGATTCCCTTGCAAGCCAGT NGG 10 8 17 8 24 0 100 5 10
    240605023658_t9vw TGCGCACTGGCTTGCAAGGGAATCAGGCTCTG(+ACTCAC)GATCCGACTTCACTTCTTATTAGCCTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605023656_7l9z AAGCCCATTCCACAGAAGTGAGTTCACATTTG(+ACTCAC)GTACCGTGAGCCTAGTCAAAAGCTTGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605023655_t6a7 TCCCTAGCTGCAAGCTTTTGACTAGGCTCACG(+ACTCAC)GTACCAAATGTGAACTCACTTCTGTGGAATG NGG 10 8 17 8 24 0 100 5 10
    240605023654_d6n1 AGCATCTTCACTCTTCTGGCCTACCAAGCTTG(+ACTCAC)GTACCTATTTCTCATCATAACTTTGGAATGT NGG 10 8 17 8 24 0 100 5 10