Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605023810_zsj0 CAGAATCTGGCACAGACTCCCAGTTCCCTCTG(+ACTCAC)GATCCTAGACAGGGCTCAATAAACTTTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605023809_ifde AGGCTACTTGTAAGTCTCAGGGGGGAATATTG(+ACTCAC)GATCCTGTCACATAGAAGCCTCCCCTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605023808_8lp7 CTGTTGCTGGAGGGGAGGCTTCTATGTGACAG(+ACTCAC)GATCCAATATTCCCCCCTGAGACTTACAAGT NGG 10 8 17 8 24 0 100 5 10
    240605023806_6iok CCTCACTCTCCTCTAAACGGTTCTAGTCCTAG(+ACTCAC)GATCCAAGGGACACTACGGGTCTCCCCATCA NGG 10 8 17 8 24 0 100 5 10
    240605023803_f6hd AGAGTTGATGGGGAGACCCGTAGTGTCCCTTG(+ACTCAC)GATCCTAGGACTAGAACCGTTTAGAGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605023801_r2y8 CCAAGGTAGCCCTCTGTTCTTGAGACCCTCGG(+ACTCAC)GTACCAAGCTACATGCCCCCTAGCAGAACTC NGG 10 8 17 8 24 0 100 5 10
    240605023800_lmxi AAATAGAGTTCTGCTAGGGGGCATGTAGCTTG(+ACTCAC)GTACCCGAGGGTCTCAAGAACAGAGGGCTAC NGG 10 8 17 8 24 0 100 5 10
    240605023758_ere8 ACCCACTATACAGCTCTATTCCTAACCAGTGG(+ACTCAC)GATCCCCAAATCCAGGCCCTCAGCAGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605023756_a61g CACGCCCCCACTGCTGAGGGCCTGGATTTGGG(+ACTCAC)GATCCCACTGGTTAGGAATAGAGCTGTATAG NGG 10 8 17 8 24 0 100 5 10
    240605023755_zpeg GCAGTCCTCCCTGTTACAGGGGCGACAGGTAG(+ACTCAC)GTACCACCTGGTTATGCCAATCTACTCATTT NGG 10 8 17 8 24 0 100 5 10