Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605023900_kzpe GAGTGTATACATCTTTCCACTAATGAGGCAGG(+ACTCAC)GTACCTTTATACCTTAGCCTTGAGATCTCTA NGG 10 8 17 8 24 0 100 5 10
    240605023859_vn1d CAACCCATAGGGTACATATTTTGTCACAAAAG(+ACTCAC)GTACCAGATGCATGCCAAACCAAACAACCTG NGG 10 8 17 8 24 0 100 5 10
    240605023857_bi62 TAATCCAGGTTGTTTGGTTTGGCATGCATCTG(+ACTCAC)GTACCTTTTGTGACAAAATATGTACCCTATG NGG 10 8 17 8 24 0 100 5 10
    240605023856_hyds TGTACAGCAGCCATCTCTTGAGGTCACACCTG(+ACTCAC)GTACCTACATAGGCAAGAAGGCTTCGGGATA NGG 10 8 17 8 24 0 100 5 10
    240605023854_0xoo CTATGTATCCCGAAGCCTTCTTGCCTATGTAG(+ACTCAC)GTACCAGGTGTGACCTCAAGAGATGGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605023853_887y TTCCCTGAGACTTGGTACTCTATGAGGTGGAG(+ACTCAC)GTACCCATCCCTGATCTGAACCCTTTTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605023851_pe11 CTAACCCAGAAAAGGGTTCAGATCAGGGATGG(+ACTCAC)GTACCTCCACCTCATAGAGTACCAAGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605023849_y2ot CAGACGCAGACGGGGCAGCAAGGACGGCCCTG(+ACTCAC)GATCCAACTCTTCAGCTACTGCTCCCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605023848_rxes GGCTTGGCTTGGGAGCAGTAGCTGAAGAGTTG(+ACTCAC)GATCCAGGGCCGTCCTTGCTGCCCCGTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605023846_5p87 TCCCCTTCATTCCATGATGAGGACACTGCCAG(+ACTCAC)GATCCAGGGGTAAAGCACTGAGCTCAGGCAG NGG 10 8 17 8 24 0 100 5 10