Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605023754_ds97 TTCTAAAATGAGTAGATTGGCATAACCAGGTG(+ACTCAC)GTACCTACCTGTCGCCCCTGTAACAGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605023752_6atg TGAAAAGGTCGAGTTCGAGGCTTCTCCATCGG(+ACTCAC)GATCCCAAAGGATGGTGACGTAGGCTTGAGT NGG 10 8 17 8 24 0 100 5 10
    240605023751_uakb ACTGGACTCAAGCCTACGTCACCATCCTTTGG(+ACTCAC)GATCCCGATGGAGAAGCCTCGAACTCGACCT NGG 10 8 17 8 24 0 100 5 10
    240605023749_oqyv AACACCGTACAAACTCTGGATAAAATGCACAG(+ACTCAC)GATCCCTCACTGCTGACATACAATGGAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605023748_deoh AGGTATAGCTCCATTGTATGTCAGCAGTGAGG(+ACTCAC)GATCCTGTGCATTTTATCCAGAGTTTGTACG NGG 10 8 17 8 24 0 100 5 10
    240605023746_adrk TGCACCTCTTCAAAAAAGAAATCTTTCTTTGG(+ACTCAC)GATCCCACATTCCCTCAGCCTTATCAACCCT NGG 10 8 17 8 24 0 100 5 10
    240605023745_htr6 GAGACAGGGTTGATAAGGCTGAGGGAATGTGG(+ACTCAC)GATCCCAAAGAAAGATTTCTTTTTTGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605023743_fw51 AAGGGCACAGAAAACTCGAAGCCCTTCCAGTG(+ACTCAC)GTACCAGCTCACTCCCTGAGGAGAGGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605023742_zkwy CCTTGACAACCCTCTCCTCAGGGAGTGAGCTG(+ACTCAC)GTACCACTGGAAGGGCTTCGAGTTTTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605023741_dzx3 AATGAAGAGGATATGGGGGAAACCCTCACGAG(+ACTCAC)GTACCCTCGGGGAAAGAGGACTGGGAGTGCA NGG 10 8 17 8 24 0 100 5 10