Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605023739_21lc ATTCCTGCACTCCCAGTCCTCTTTCCCCGAGG(+ACTCAC)GTACCTCGTGAGGGTTTCCCCCATATCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605023737_aqlk CTGTGGCCTGCAAGGCCCATCTCAGGTTTGGG(+ACTCAC)GATCCCCCAGTCCCTATGATCAGTGTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605023735_7jzl GGATACCCCAACACTGATCATAGGGACTGGGG(+ACTCAC)GATCCCCAAACCTGAGATGGGCCTTGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605023733_8m78 GGGAGGAAATGAAAATTGGCTCAGAGACGGAG(+ACTCAC)GTACCTCAAAAAAAAAAAAAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605023732_jbgq ATTTCTTTTTTTTTTTTTTTTTTTTTTTTGAG(+ACTCAC)GTACCTCCGTCTCTGAGCCAATTTTCATTTC NGG 10 8 17 8 24 0 100 5 10
    240605023731_mlu6 GGGAGGAAATGAAAATTGGCTCAGAGACGGAG(+ACTCAC)GTACCTCAAAAAAAAAAAAAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605023729_8zgj ATTTCTTTTTTTTTTTTTTTTTTTTTTTTGAG(+ACTCAC)GTACCTCCGTCTCTGAGCCAATTTTCATTTC NGG 10 8 17 8 24 0 100 5 10
    240605023728_9aaf TTCTACATTTGTTCCTGGTGATGAGATTGCTG(+ACTCAC)GATCCTAGATAGTTCCTCGAAGTGGACCTTC NGG 10 8 17 8 24 0 100 5 10
    240605023727_kx5d ACTGAGAAGGTCCACTTCGAGGAACTATCTAG(+ACTCAC)GATCCAGCAATCTCATCACCAGGAACAAATG NGG 10 8 17 8 24 0 100 5 10
    240605023726_zppu ATTCTTCAAACATGACTGAGACTGACTCTCAG(+ACTCAC)GATCCTGCTCAGCGCAGGTTCACAGAGGAGC NGG 10 8 17 8 24 0 100 5 10