Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605023914_jgjc GATCTCTGCTTCCTTTCTAGATTCTCAGAGGG(+ACTCAC)GTACCCCCGCCCCTGAAGTGTAGAGGTCAAG NGG 10 8 17 8 24 0 100 5 10
    240605023913_1u02 TTCCCCCAAACACACATCGCAGAAGAAACATG(+ACTCAC)GTACCATTAGCAAAGCTCTGAAACTTTACAG NGG 10 8 17 8 24 0 100 5 10
    240605023912_y4wo AGGATCTGTAAAGTTTCAGAGCTTTGCTAATG(+ACTCAC)GTACCATGTTTCTTCTGCGATGTGTGTTTGG NGG 10 8 17 8 24 0 100 5 10
    240605023910_1c1f AAGGTAGGCAACAGAGTGGTTTTGGGCACGGG(+ACTCAC)GTACCTCAACTTCGAGGACAGCAAAAACAGC NGG 10 8 17 8 24 0 100 5 10
    240605023908_ls6v CTGAAGCTGTTTTTGCTGTCCTCGAAGTTGAG(+ACTCAC)GTACCCCGTGCCCAAAACCACTCTGTTGCCT NGG 10 8 17 8 24 0 100 5 10
    240605023907_ivpk AATAGGGCCCAGAGGAAAAAAAAATGTGTGAG(+ACTCAC)GATCCACAATGGGTACTTGTGTTTCCTACTG NGG 10 8 17 8 24 0 100 5 10
    240605023906_w3ho CCTTGCAGTAGGAAACACAAGTACCCATTGTG(+ACTCAC)GATCCTCACACATTTTTTTTTCCTCTGGGCC NGG 10 8 17 8 24 0 100 5 10
    240605023905_togg GCTTGCTGAGCCCCTTGCAATTAGGAAGACAG(+ACTCAC)GTACCCATTGTGGGTACTCACACATTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605023903_489h CCAGAGAGAAAATGTGTGAGTACCCACAATGG(+ACTCAC)GTACCTGTCTTCCTAATTGCAAGGGGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605023902_zelu CTCCCTAGAGATCTCAAGGCTAAGGTATAAAG(+ACTCAC)GTACCCTGCCTCATTAGTGGAAAGATGTATA NGG 10 8 17 8 24 0 100 5 10