Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605024017_5u3e TGCTTTCCCCAGGCCTCCAGCCATGGTTCCAG(+ACTCAC)GTACCCACCTTGGCTCTCATTAACCCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605024015_0tf7 TGGCCTCCCAACCAAGGGAGCCATCATGGTGG(+ACTCAC)GTACCAAGAAAGGCAAAGCACTTGGGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605024014_j85k AAAAAAAAGCCCCAAGTGCTTTGCCTTTCTTG(+ACTCAC)GTACCCACCATGATGGCTCCCTTGGTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605024013_q5rj TGTGGCCTCAGGCCTCAGTTCTTGGCCAACTG(+ACTCAC)GTACCTGAATGTCTTGTTCTCATTTAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605024012_7wbk AAGCTGCCCCTAAATGAGAACAAGACATTCAG(+ACTCAC)GTACCAGTTGGCCAAGAACTGAGGCCTGAGG NGG 10 8 17 8 24 0 100 5 10
    240605024010_qlk4 CAGTCACAGCGTGCCAGGGACTCAAGGAGAGG(+ACTCAC)GTACCTGGAAGACTGTATGTCCTGAGGATAA NGG 10 8 17 8 24 0 100 5 10
    240605024008_mdgn AGCCCTTATCCTCAGGACATACAGTCTTCCAG(+ACTCAC)GTACCCTCTCCTTGAGTCCCTGGCACGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605024006_j2f8 CAGTCACAGCGTGCCAGGGACTCAAGGAGAGG(+ACTCAC)GTACCTGGAAGACTGTATGTCCTGAGGATAA NGG 10 8 17 8 24 0 100 5 10
    240605024004_rrtu AGCCCTTATCCTCAGGACATACAGTCTTCCAG(+ACTCAC)GTACCCTCTCCTTGAGTCCCTGGCACGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605024003_y15l CCTCCTTGGAGGATGTACTGTGATCATTCCTG(+ACTCAC)GTACCTTAGGGCCAATCCCTAAGTGTGGCTT NGG 10 8 17 8 24 0 100 5 10