Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605024121_cgfd GTCAGATGCTCTATGTCTAGGCTGTCTGGGTG(+ACTCAC)GATCCCTGATTCCAGAGATGGTCAGAGACTT NGG 10 8 17 8 24 0 100 5 10
    240605024119_b675 AGCTACATCCTAAGTTCTGAGTGAGGGGGTAG(+ACTCAC)GATCCACAGGAAGAAATAAGTCATGAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605024118_l5bo CTCCCCAAGCTCATGACTTATTTCTTCCTGTG(+ACTCAC)GATCCTACCCCCTCACTCAGAACTTAGGATG NGG 10 8 17 8 24 0 100 5 10
    240605024117_b5o7 CCCAGCCCAGCCGCCACCAGCCTCTCTGATTG(+ACTCAC)GATCCCCTTAACTGCATGCTCTTGAACCCCA NGG 10 8 17 8 24 0 100 5 10
    240605024115_g14r CCCCATGGGGTTCAAGAGCATGCAGTTAAGGG(+ACTCAC)GATCCAATCAGAGAGGCTGGTGGCGGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605024113_tfs6 GGCAAGTCTGCCTAGCTGTGCTCTGAGGACAG(+ACTCAC)GATCCTAACTGACAGCCTACACTTTGTAGAT NGG 10 8 17 8 24 0 100 5 10
    240605024112_vi3z TGTTAATCTACAAAGTGTAGGCTGTCAGTTAG(+ACTCAC)GATCCTGTCCTCAGAGCACAGCTAGGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240605024111_0631 TCCTCAAAGCTAAAAACGGCTGCCTGTGTGTG(+ACTCAC)GATCCTGCTCTCTCTTGAGGTCGTTACCTCA NGG 10 8 17 8 24 0 100 5 10
    240605024110_dwh7 CAGTCTGAGGTAACGACCTCAAGAGAGAGCAG(+ACTCAC)GATCCACACACAGGCAGCCGTTTTTAGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605024108_9r6o CATTTCCTCTGAGAACCGCTGAAGATTAGTAG(+ACTCAC)GATCCCCACATCTCCCAGTTAAAGCCCAGTT NGG 10 8 17 8 24 0 100 5 10