Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605024050_7or3 TTCCCTACTTAGGAAGATGCAGGATTCTGCAG(+ACTCAC)GATCCCAGAATGTTAAATAGCCATGCTGTTT NGG 10 8 17 8 24 0 100 5 10
    240605024048_dv5w GGAGGAGGCAGCCTTCAGGCGACAGGCGGGAG(+ACTCAC)GTACCTTCAGTCAGGGGGCATAGGCAGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605024047_ivee GCCTGCCCTCTGCCTATGCCCCCTGACTGAAG(+ACTCAC)GTACCTCCCGCCTGTCGCCTGAAGGCTGCCT NGG 10 8 17 8 24 0 100 5 10
    240605024045_v6b1 TGCCACCCCTTCCCTTATCAGGGACAGTATGG(+ACTCAC)GTACCTCAGGTAACCCCAATTTCTGGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605024043_fr9j AAGTCTCAGGCCAGAAATTGGGGTTACCTGAG(+ACTCAC)GTACCCATACTGTCCCTGATAAGGGAAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605024042_tast AGTCATCTCTACCACCAGTTCCAGAGAATGTG(+ACTCAC)GTACCCTCTTCTGACCTCTGGGCACAAGGTA NGG 10 8 17 8 24 0 100 5 10
    240605024040_lyv2 ATTCATACCTTGTGCCCAGAGGTCAGAAGAGG(+ACTCAC)GTACCACATTCTCTGGAACTGGTGGTAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605024039_75gj CTCTGAGCCAGCCCCCACAGCTGAGCTGCTCG(+ACTCAC)GTACCTGACATGGGGTCTTCTGCTGCATTTT NGG 10 8 17 8 24 0 100 5 10
    240605024038_v8pt AAGCCAAAATGCAGCAGAAGACCCCATGTCAG(+ACTCAC)GTACCGAGCAGCTCAGCTGTGGGGGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605024036_8nad CAGGACAACTCCTGCAGCACTGGGCTGTTGGG(+ACTCAC)GATCCTGGGCACCCCTCTCATGGTGGCGGTG NGG 10 8 17 8 24 0 100 5 10