Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605024139_bwsj AACATGGCTTTTTTGGATCTCCTCTGTGTTGG(+ACTCAC)GTACCATATCCTCTTTGCTACCCTTCTATTT NGG 10 8 17 8 24 0 100 5 10
    240605024136_y8vo GCCAGAAGACAACCTTGGCTCTCATTCCCCAG(+ACTCAC)GTACCATCCAGCTTGGTCTTTAAGATGGGAT NGG 10 8 17 8 24 0 100 5 10
    240605024134_j8gm AAGAGATCCCATCTTAAAGACCAAGCTGGATG(+ACTCAC)GTACCTGGGGAATGAGAGCCAAGGTTGTCTT NGG 10 8 17 8 24 0 100 5 10
    240605024133_ceif ATAGCCACTATGCCTCAAGATGTCCATACCAG(+ACTCAC)GATCCAGGTCTGAAACTTCTATCTCCCAGCC NGG 10 8 17 8 24 0 100 5 10
    240605024130_dlh5 CTGGAGGCTGGGAGATAGAAGTTTCAGACCTG(+ACTCAC)GATCCTGGTATGGACATCTTGAGGCATAGTG NGG 10 8 17 8 24 0 100 5 10
    240605024129_4bbv TGAGACAACTCAGTTGTCATGTCAGGGGGAAG(+ACTCAC)GATCCTATTTCAGACTGCCTCCTGTGTGCCT NGG 10 8 17 8 24 0 100 5 10
    240605024128_zmk8 GGTAGAGGCACACAGGAGGCAGTCTGAAATAG(+ACTCAC)GATCCTTCCCCCTGACATGACAACTGAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605024127_uskj GAAGTGTCCATGAAGACTGCAACACAGCATTG(+ACTCAC)GATCCCCCTGAGCTGCAGTTACAGACATTTG NGG 10 8 17 8 24 0 100 5 10
    240605024124_88ez GCTCACAAATGTCTGTAACTGCAGCTCAGGGG(+ACTCAC)GATCCAATGCTGTGTTGCAGTCTTCATGGAC NGG 10 8 17 8 24 0 100 5 10
    240605024123_w7jl CCATAAAGTCTCTGACCATCTCTGGAATCAGG(+ACTCAC)GATCCACCCAGACAGCCTAGACATAGAGCAT NGG 10 8 17 8 24 0 100 5 10